An innovative non-invasive technique for subcutaneous tumour measurements

Juan Delgado San Martin, Beate Ehrhardt, Marcin Paczkowski, Sean Hackett, Andrew Smith, Wajahat Waraich, James Klatzow, Adeala Zabair, Anna Chabokdast, Leonardo Rubio-Navarro, Amar Rahi, Zena Wilson

Research output: Contribution to journalArticle

Abstract

INTRODUCTION: In oncological drug development, animal studies continue to play a central role in which the volume of subcutaneous tumours is monitored to assess the efficacy of new drugs. The tumour volume is estimated by taking the volume to be that of a regular spheroid with the same dimensions. However, this method is subjective, insufficiently traceable, and is subject to error in the accuracy of volume estimates as tumours are frequently irregular.

METHODS & RESULTS: This paper reviews the standard technique for tumour volume assessment, calliper measurements, by conducting a statistical review of a large dataset consisting of 2,500 tumour volume measurements from 1,600 mice by multiple operators across 6 mouse strains and 20 tumour models. Additionally, we explore the impact of six different tumour morphologies on volume estimation and the detection of treatment effects using a computational tumour growth model. Finally, we propose an alternative method to callipers for estimating volume-BioVolumeTM, a 3D scanning technique. BioVolume simultaneously captures both stereo RGB (Red, Green and Blue) images from different light sources and infrared thermal images of the tumour in under a second. It then detects the tumour region automatically and estimates the tumour volume in under a minute. Furthermore, images can be processed in parallel within the cloud and so the time required to process multiple images is similar to that required for a single image. We present data of a pre-production unit test consisting of 297 scans from over 120 mice collected by four different operators.

CONCLUSION: This work demonstrates that it is possible to record tumour measurements in a rapid minimally invasive, morphology-independent way, and with less human-bias compared to callipers, whilst also improving data traceability. Furthermore, the images collected by BioVolume may be useful, for example, as a source of biomarkers for animal welfare and secondary drug toxicity / efficacy.

Original languageEnglish
Article numbere0216690
Pages (from-to)1-14
Number of pages14
JournalPLoS ONE
Volume14
Issue number10
DOIs
Publication statusPublished - 14 Oct 2019

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Martin, J. D. S., Ehrhardt, B., Paczkowski, M., Hackett, S., Smith, A., Waraich, W., ... Wilson, Z. (2019). An innovative non-invasive technique for subcutaneous tumour measurements. PLoS ONE, 14(10), 1-14. [e0216690]. https://doi.org/10.1371/journal.pone.0216690

An innovative non-invasive technique for subcutaneous tumour measurements. / Martin, Juan Delgado San; Ehrhardt, Beate; Paczkowski, Marcin; Hackett, Sean; Smith, Andrew; Waraich, Wajahat; Klatzow, James; Zabair, Adeala; Chabokdast, Anna; Rubio-Navarro, Leonardo; Rahi, Amar; Wilson, Zena.

In: PLoS ONE, Vol. 14, No. 10, e0216690, 14.10.2019, p. 1-14.

Research output: Contribution to journalArticle

Martin, JDS, Ehrhardt, B, Paczkowski, M, Hackett, S, Smith, A, Waraich, W, Klatzow, J, Zabair, A, Chabokdast, A, Rubio-Navarro, L, Rahi, A & Wilson, Z 2019, 'An innovative non-invasive technique for subcutaneous tumour measurements', PLoS ONE, vol. 14, no. 10, e0216690, pp. 1-14. https://doi.org/10.1371/journal.pone.0216690
Martin JDS, Ehrhardt B, Paczkowski M, Hackett S, Smith A, Waraich W et al. An innovative non-invasive technique for subcutaneous tumour measurements. PLoS ONE. 2019 Oct 14;14(10):1-14. e0216690. https://doi.org/10.1371/journal.pone.0216690
Martin, Juan Delgado San ; Ehrhardt, Beate ; Paczkowski, Marcin ; Hackett, Sean ; Smith, Andrew ; Waraich, Wajahat ; Klatzow, James ; Zabair, Adeala ; Chabokdast, Anna ; Rubio-Navarro, Leonardo ; Rahi, Amar ; Wilson, Zena. / An innovative non-invasive technique for subcutaneous tumour measurements. In: PLoS ONE. 2019 ; Vol. 14, No. 10. pp. 1-14.
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