An increase in the speed of response, and sensitivity, of Avena fatua aleurone layers and protoplasts to gibberellin

S J Smith, R Hooley

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Abstract

In wild oat (Avena fatua) aleurone protoplasts and layers, a-amylase induction by gibberellin is very slow compared with other members of the Gramineae. Wild oat aleurone protoplasts for example do not secrete a-amylase until approximately 72 hours after treatment with gibberellin while in aleurone layers or protoplasts from wheat or barley this response takes between 8 and 24 hours. In this study, we demonstrate that the duration of the lag phase in A. fatua aleurone protoplasts can be reduced substantially by incubating the protoplasts in the absence of hormone for up to 4 days before treating with gibberellin. RNA gel blot analysis revealed substantial induction of alpha-amylass mRNA within 14 hours of gibberellin treatment in aged aleurone protoplasts. A reduction in the duration of the lag phase is also observed in intact wild oat aleurone layers that have been incubated in the absence of hormone for 4 days prior to gibberellin treatment. Interestingly, this effect is only observed in aleurones from which the endosperm has been removed. Dose response analysis revealed that the concentration of gibberellin required to induce a maximum response was approximately one order of magnitude lower in the 4 day-old tissue compared with freshly isolated material. These observations suggest that the long duration of the lag phase of freshly isolated aleurone layers and protoplasts of wild oat is due in part to gibberellin independent events.
LanguageEnglish
Pages355-360
Number of pages6
JournalJournal of Plant Physiology
Volume159
Issue number4
StatusPublished - 2002

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Gibberellins
Avena fatua
aleurone layer
Protoplasts
gibberellins
protoplasts
oats
Amylases
amylases
duration
hormones
Hormones
Endosperm
Hordeum
Poaceae
Avena
Triticum
endosperm
dose response
Gels

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An increase in the speed of response, and sensitivity, of Avena fatua aleurone layers and protoplasts to gibberellin. / Smith, S J; Hooley, R.

In: Journal of Plant Physiology, Vol. 159, No. 4, 2002, p. 355-360.

Research output: Contribution to journalArticle

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abstract = "In wild oat (Avena fatua) aleurone protoplasts and layers, a-amylase induction by gibberellin is very slow compared with other members of the Gramineae. Wild oat aleurone protoplasts for example do not secrete a-amylase until approximately 72 hours after treatment with gibberellin while in aleurone layers or protoplasts from wheat or barley this response takes between 8 and 24 hours. In this study, we demonstrate that the duration of the lag phase in A. fatua aleurone protoplasts can be reduced substantially by incubating the protoplasts in the absence of hormone for up to 4 days before treating with gibberellin. RNA gel blot analysis revealed substantial induction of alpha-amylass mRNA within 14 hours of gibberellin treatment in aged aleurone protoplasts. A reduction in the duration of the lag phase is also observed in intact wild oat aleurone layers that have been incubated in the absence of hormone for 4 days prior to gibberellin treatment. Interestingly, this effect is only observed in aleurones from which the endosperm has been removed. Dose response analysis revealed that the concentration of gibberellin required to induce a maximum response was approximately one order of magnitude lower in the 4 day-old tissue compared with freshly isolated material. These observations suggest that the long duration of the lag phase of freshly isolated aleurone layers and protoplasts of wild oat is due in part to gibberellin independent events.",
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N2 - In wild oat (Avena fatua) aleurone protoplasts and layers, a-amylase induction by gibberellin is very slow compared with other members of the Gramineae. Wild oat aleurone protoplasts for example do not secrete a-amylase until approximately 72 hours after treatment with gibberellin while in aleurone layers or protoplasts from wheat or barley this response takes between 8 and 24 hours. In this study, we demonstrate that the duration of the lag phase in A. fatua aleurone protoplasts can be reduced substantially by incubating the protoplasts in the absence of hormone for up to 4 days before treating with gibberellin. RNA gel blot analysis revealed substantial induction of alpha-amylass mRNA within 14 hours of gibberellin treatment in aged aleurone protoplasts. A reduction in the duration of the lag phase is also observed in intact wild oat aleurone layers that have been incubated in the absence of hormone for 4 days prior to gibberellin treatment. Interestingly, this effect is only observed in aleurones from which the endosperm has been removed. Dose response analysis revealed that the concentration of gibberellin required to induce a maximum response was approximately one order of magnitude lower in the 4 day-old tissue compared with freshly isolated material. These observations suggest that the long duration of the lag phase of freshly isolated aleurone layers and protoplasts of wild oat is due in part to gibberellin independent events.

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