An in vitro study of electrically active hydroxyapatite-barium titanate ceramics using Saos-2 cells

Frances Baxter, Irene G Turner, Christopher R Bowen, J P Gittings, Julian B Chaudhuri

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Electrically active ceramics are of interest as bone graft substitute materials. This study investigated the ferroelectric properties of hydroxyapatite-barium titanate (HABT) composites and the behaviour of osteoblast-like cells seeded on their surfaces. A piezoelectric coefficient (d33) of 57.8 pCN−1 was observed in HABT discs prepared for cell culture. The attachment, proliferation, viability, morphology and metabolic activity of cells cultured on unpoled HABT were comparable to those observed on commercially available hydroxyapatite at all time points. No indication of the cytotoxicity of HABT was detected. At one day after seeding, cell attachment was modified on both the positive and negative surfaces of poled HABT. After longer incubations, all parameters observed were comparable on poled and unpoled ceramics. The results indicate that HABT ceramics are biocompatible in the short term in vitro and that further investigation of cell responses to these materials under mechanical load and at longer incubation times is warranted.
Original languageEnglish
Pages (from-to)1697-1708
Number of pages12
JournalJournal of Materials Science: Materials in Medicine
Volume20
Issue number8
Early online date24 Mar 2009
DOIs
Publication statusPublished - Aug 2009

Fingerprint

Barium titanate
Ceramics
Hydroxyapatite
Cells
Bone Substitutes
Osteoblasts
Durapatite
Cytotoxicity
Cell culture
Grafts
Ferroelectric materials
In Vitro Techniques
barium hydroxyapatite
Cultured Cells
Bone
Cell Culture Techniques
Transplants
Composite materials

Cite this

An in vitro study of electrically active hydroxyapatite-barium titanate ceramics using Saos-2 cells. / Baxter, Frances; Turner, Irene G; Bowen, Christopher R; Gittings, J P; Chaudhuri, Julian B.

In: Journal of Materials Science: Materials in Medicine, Vol. 20, No. 8, 08.2009, p. 1697-1708.

Research output: Contribution to journalArticle

Baxter, Frances ; Turner, Irene G ; Bowen, Christopher R ; Gittings, J P ; Chaudhuri, Julian B. / An in vitro study of electrically active hydroxyapatite-barium titanate ceramics using Saos-2 cells. In: Journal of Materials Science: Materials in Medicine. 2009 ; Vol. 20, No. 8. pp. 1697-1708.
@article{11ffd214f1f24f999ab110edf4537d42,
title = "An in vitro study of electrically active hydroxyapatite-barium titanate ceramics using Saos-2 cells",
abstract = "Electrically active ceramics are of interest as bone graft substitute materials. This study investigated the ferroelectric properties of hydroxyapatite-barium titanate (HABT) composites and the behaviour of osteoblast-like cells seeded on their surfaces. A piezoelectric coefficient (d33) of 57.8 pCN−1 was observed in HABT discs prepared for cell culture. The attachment, proliferation, viability, morphology and metabolic activity of cells cultured on unpoled HABT were comparable to those observed on commercially available hydroxyapatite at all time points. No indication of the cytotoxicity of HABT was detected. At one day after seeding, cell attachment was modified on both the positive and negative surfaces of poled HABT. After longer incubations, all parameters observed were comparable on poled and unpoled ceramics. The results indicate that HABT ceramics are biocompatible in the short term in vitro and that further investigation of cell responses to these materials under mechanical load and at longer incubation times is warranted.",
author = "Frances Baxter and Turner, {Irene G} and Bowen, {Christopher R} and Gittings, {J P} and Chaudhuri, {Julian B}",
year = "2009",
month = "8",
doi = "10.1007/s10856-009-3734-0",
language = "English",
volume = "20",
pages = "1697--1708",
journal = "Journal of Materials Science: Materials in Medicine",
issn = "0957-4530",
publisher = "Springer Netherlands",
number = "8",

}

TY - JOUR

T1 - An in vitro study of electrically active hydroxyapatite-barium titanate ceramics using Saos-2 cells

AU - Baxter, Frances

AU - Turner, Irene G

AU - Bowen, Christopher R

AU - Gittings, J P

AU - Chaudhuri, Julian B

PY - 2009/8

Y1 - 2009/8

N2 - Electrically active ceramics are of interest as bone graft substitute materials. This study investigated the ferroelectric properties of hydroxyapatite-barium titanate (HABT) composites and the behaviour of osteoblast-like cells seeded on their surfaces. A piezoelectric coefficient (d33) of 57.8 pCN−1 was observed in HABT discs prepared for cell culture. The attachment, proliferation, viability, morphology and metabolic activity of cells cultured on unpoled HABT were comparable to those observed on commercially available hydroxyapatite at all time points. No indication of the cytotoxicity of HABT was detected. At one day after seeding, cell attachment was modified on both the positive and negative surfaces of poled HABT. After longer incubations, all parameters observed were comparable on poled and unpoled ceramics. The results indicate that HABT ceramics are biocompatible in the short term in vitro and that further investigation of cell responses to these materials under mechanical load and at longer incubation times is warranted.

AB - Electrically active ceramics are of interest as bone graft substitute materials. This study investigated the ferroelectric properties of hydroxyapatite-barium titanate (HABT) composites and the behaviour of osteoblast-like cells seeded on their surfaces. A piezoelectric coefficient (d33) of 57.8 pCN−1 was observed in HABT discs prepared for cell culture. The attachment, proliferation, viability, morphology and metabolic activity of cells cultured on unpoled HABT were comparable to those observed on commercially available hydroxyapatite at all time points. No indication of the cytotoxicity of HABT was detected. At one day after seeding, cell attachment was modified on both the positive and negative surfaces of poled HABT. After longer incubations, all parameters observed were comparable on poled and unpoled ceramics. The results indicate that HABT ceramics are biocompatible in the short term in vitro and that further investigation of cell responses to these materials under mechanical load and at longer incubation times is warranted.

UR - http://www.scopus.com/inward/record.url?scp=68249109133&partnerID=8YFLogxK

UR - http://dx.doi.org/10.1007/s10856-009-3734-0

U2 - 10.1007/s10856-009-3734-0

DO - 10.1007/s10856-009-3734-0

M3 - Article

VL - 20

SP - 1697

EP - 1708

JO - Journal of Materials Science: Materials in Medicine

JF - Journal of Materials Science: Materials in Medicine

SN - 0957-4530

IS - 8

ER -