Abstract
Albugo candida is an obligate oomycete pathogen that infects many plants in the Brassicaceae family. We resequenced the genome of isolate Ac2V using PacBio long reads and constructed an assembly augmented by Illumina reads. The Ac2VPB genome assembly is 10% larger and more contiguous compared with a previous version. Our annotation of the new assembly, aided by RNA-sequencing information, revealed a 175% expansion (40 to 110) in the CHxC effector class, which we redefined as “CCG” based on motif analysis. This class of effectors consist of arrays of phylogenetically related paralogs residing in gene sparse regions, and shows signatures of positive selection and presence/absence polymorphism. This work provides a resource that allows the dissection of the genomic components underlying A. candida adaptation and, particularly, the role of CCG effectors in virulence and avirulence on different hosts.
| Original language | English |
|---|---|
| Pages (from-to) | 39-48 |
| Number of pages | 10 |
| Journal | Molecular plant-microbe interactions : MPMI |
| Volume | 35 |
| Issue number | 1 |
| Early online date | 17 Dec 2021 |
| DOIs | |
| Publication status | Published - 1 Jan 2022 |
Bibliographical note
Funding Information:Funding: Support was provided by the Biotechnology and Biological Sciences Research Council (grant BB/L011646/1 to V. Cevik and grant BB/ M003809/1 to O. J. Furzer), European Molecular Biology Organization (long-term fellowship ALTF-842-2015 to A. Redkar), and H2020 European Research Council (grant 233376 to K. Bailey and J. D. G. Jones). The Sains-bury Laboratory is supported by the Gatsby Charitable Foundation.
Publisher Copyright:
Copyright © 2021 The Author(s).
Keywords
- Albugo candida
- Biotrophy
- Effectors
- Genome
- Genomics
- Oomycete
- Oomycete–plant interactions
- PacBio
- Population biology
ASJC Scopus subject areas
- Physiology
- Agronomy and Crop Science