An electrochemical study of enzymatic oligonucleotide digestion

S C Hillier; Christopher G Frost; A Toby A Jenkins; H T Braven; R W Keay; Stephen E Flower; J M Clarkson

doi:10.1016/j.bioelechem.2003.10.028

An electrochemical study of enzymatic oligonucleotide digestion

S C Hillier, Christopher G Frost, A Toby A Jenkins, H T Braven, R W Keay, Stephen E Flower, J M Clarkson

Department of Chemistry

Molecular Sensing PLC

Research output: Contribution to journal › Article › peer-review

36 Citations (SciVal)

Abstract

This paper describes the synthesis and application of a novel ferrocene (Fc) label that can be efficiently attached to oligonucleotides. We demonstrate how pulse electrochemical methods can be used to measure very low concentrations of ferrocene label and, importantly, show good electroanalytical discrimination between a labelled oligonucleotide and an enzyme digested labelled oligonucleotide, in which the ferrocene label nucleotide conjugate has been released. Real time in situ analysis gives a much greater understanding of the process. Potential applications include the detection of specific nucleic acid sequences and measurement of nuclease activity.

Original language	English
Pages (from-to)	307-310
Number of pages	4
Journal	Bioelectrochemistry
Volume	63
Issue number	1-2
Early online date	18 Feb 2004
DOIs	https://doi.org/10.1016/j.bioelechem.2003.10.028
Publication status	Published - 30 Jun 2004

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10.1016/j.bioelechem.2003.10.028

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AU - Hillier, S C

AU - Frost, Christopher G

AU - Jenkins, A Toby A

AU - Braven, H T

AU - Keay, R W

AU - Flower, Stephen E

AU - Clarkson, J M

PY - 2004/6/30

Y1 - 2004/6/30

N2 - This paper describes the synthesis and application of a novel ferrocene (Fc) label that can be efficiently attached to oligonucleotides. We demonstrate how pulse electrochemical methods can be used to measure very low concentrations of ferrocene label and, importantly, show good electroanalytical discrimination between a labelled oligonucleotide and an enzyme digested labelled oligonucleotide, in which the ferrocene label nucleotide conjugate has been released. Real time in situ analysis gives a much greater understanding of the process. Potential applications include the detection of specific nucleic acid sequences and measurement of nuclease activity.

AB - This paper describes the synthesis and application of a novel ferrocene (Fc) label that can be efficiently attached to oligonucleotides. We demonstrate how pulse electrochemical methods can be used to measure very low concentrations of ferrocene label and, importantly, show good electroanalytical discrimination between a labelled oligonucleotide and an enzyme digested labelled oligonucleotide, in which the ferrocene label nucleotide conjugate has been released. Real time in situ analysis gives a much greater understanding of the process. Potential applications include the detection of specific nucleic acid sequences and measurement of nuclease activity.

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DO - 10.1016/j.bioelechem.2003.10.028

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VL - 63

SP - 307

EP - 310

JO - Bioelectrochemistry

JF - Bioelectrochemistry

IS - 1-2

ER -

An electrochemical study of enzymatic oligonucleotide digestion

Abstract

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