TY - JOUR
T1 - An electrochemical sensor concept for the detection of virulence factors from Staphylococcus aureus and Pseudomonas aeruginosa
AU - Thet, Naing Tun
AU - Jenkins, A. Toby A.
PY - 2015/10
Y1 - 2015/10
N2 - This work presents an electrochemical sensor concept for bacterial toxin detection using biomimetic lipid vesicles containing potassium ferricyanide. The toxin mediated release of redox couples from the vesicles was quantified by measuring the redox current on screen printed electrodes, and the detection limits to rhamnolipid, delta toxin and bacterial supernatants from clinical wound pathogens were examined. Overall detection limits of both redox and carboxyfluorescein containing vesicles were one order of magnitude lower than the cytotoxic dose of studied toxins to T lymphocytes.
AB - This work presents an electrochemical sensor concept for bacterial toxin detection using biomimetic lipid vesicles containing potassium ferricyanide. The toxin mediated release of redox couples from the vesicles was quantified by measuring the redox current on screen printed electrodes, and the detection limits to rhamnolipid, delta toxin and bacterial supernatants from clinical wound pathogens were examined. Overall detection limits of both redox and carboxyfluorescein containing vesicles were one order of magnitude lower than the cytotoxic dose of studied toxins to T lymphocytes.
KW - Delta toxin
KW - Lipid vesicles
KW - Potassium ferricyanide
KW - Pseudomonas aeruginosa
KW - Rhamnolipid
KW - Staphylococcus aureus
UR - http://www.sciencedirect.com/science/article/pii/S1388248115000028
UR - http://dx.doi.org/10.1016/j.elecom.2015.01.001
U2 - 10.1016/j.elecom.2015.01.001
DO - 10.1016/j.elecom.2015.01.001
M3 - Article
VL - 59
SP - 104
EP - 108
JO - Electrochemistry Communications
JF - Electrochemistry Communications
SN - 1388-2481
ER -