An electrochemical sensor concept for the detection of virulence factors from Staphylococcus aureus and Pseudomonas aeruginosa

Naing Tun Thet; A. Toby A. Jenkins

doi:10.1016/j.elecom.2015.01.001

An electrochemical sensor concept for the detection of virulence factors from Staphylococcus aureus and Pseudomonas aeruginosa

Naing Tun Thet, A. Toby A. Jenkins

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Abstract

This work presents an electrochemical sensor concept for bacterial toxin detection using biomimetic lipid vesicles containing potassium ferricyanide. The toxin mediated release of redox couples from the vesicles was quantified by measuring the redox current on screen printed electrodes, and the detection limits to rhamnolipid, delta toxin and bacterial supernatants from clinical wound pathogens were examined. Overall detection limits of both redox and carboxyfluorescein containing vesicles were one order of magnitude lower than the cytotoxic dose of studied toxins to T lymphocytes.

Original language	English
Pages (from-to)	104-108
Journal	Electrochemistry Communications
Volume	59
Early online date	9 Jan 2015
DOIs	https://doi.org/10.1016/j.elecom.2015.01.001
Publication status	Published - Oct 2015

Keywords

Delta toxin
Lipid vesicles
Potassium ferricyanide
Pseudomonas aeruginosa
Rhamnolipid
Staphylococcus aureus

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10.1016/j.elecom.2015.01.001

An electrochemical sensor concept for the detection of bacterial virulence factors from S. aureus and P. aeruginosa (Thet & Jenkins).PDF
Version of Record available at: http://dx.doi.org/10.1016/j.elecom.2015.01.001
Accepted author manuscript, 474 KBLicence: CC BY-NC-ND

Cite this

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title = "An electrochemical sensor concept for the detection of virulence factors from Staphylococcus aureus and Pseudomonas aeruginosa",

abstract = "This work presents an electrochemical sensor concept for bacterial toxin detection using biomimetic lipid vesicles containing potassium ferricyanide. The toxin mediated release of redox couples from the vesicles was quantified by measuring the redox current on screen printed electrodes, and the detection limits to rhamnolipid, delta toxin and bacterial supernatants from clinical wound pathogens were examined. Overall detection limits of both redox and carboxyfluorescein containing vesicles were one order of magnitude lower than the cytotoxic dose of studied toxins to T lymphocytes.",

keywords = "Delta toxin, Lipid vesicles, Potassium ferricyanide, Pseudomonas aeruginosa, Rhamnolipid, Staphylococcus aureus",

author = "Thet, {Naing Tun} and Jenkins, {A. Toby A.}",

year = "2015",

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language = "English",

volume = "59",

pages = "104--108",

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T1 - An electrochemical sensor concept for the detection of virulence factors from Staphylococcus aureus and Pseudomonas aeruginosa

AU - Thet, Naing Tun

AU - Jenkins, A. Toby A.

PY - 2015/10

Y1 - 2015/10

N2 - This work presents an electrochemical sensor concept for bacterial toxin detection using biomimetic lipid vesicles containing potassium ferricyanide. The toxin mediated release of redox couples from the vesicles was quantified by measuring the redox current on screen printed electrodes, and the detection limits to rhamnolipid, delta toxin and bacterial supernatants from clinical wound pathogens were examined. Overall detection limits of both redox and carboxyfluorescein containing vesicles were one order of magnitude lower than the cytotoxic dose of studied toxins to T lymphocytes.

AB - This work presents an electrochemical sensor concept for bacterial toxin detection using biomimetic lipid vesicles containing potassium ferricyanide. The toxin mediated release of redox couples from the vesicles was quantified by measuring the redox current on screen printed electrodes, and the detection limits to rhamnolipid, delta toxin and bacterial supernatants from clinical wound pathogens were examined. Overall detection limits of both redox and carboxyfluorescein containing vesicles were one order of magnitude lower than the cytotoxic dose of studied toxins to T lymphocytes.

KW - Delta toxin

KW - Lipid vesicles

KW - Potassium ferricyanide

KW - Pseudomonas aeruginosa

KW - Rhamnolipid

KW - Staphylococcus aureus

UR - http://www.sciencedirect.com/science/article/pii/S1388248115000028

UR - http://dx.doi.org/10.1016/j.elecom.2015.01.001

U2 - 10.1016/j.elecom.2015.01.001

DO - 10.1016/j.elecom.2015.01.001

M3 - Article

SN - 1388-2481

VL - 59

SP - 104

EP - 108

JO - Electrochemistry Communications

JF - Electrochemistry Communications

ER -

An electrochemical sensor concept for the detection of virulence factors from Staphylococcus aureus and Pseudomonas aeruginosa

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Keywords

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Encapsulated Phage for Treatment of Burns and Wound Site Infections

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An electrochemical sensor concept for the detection of virulence factors from Staphylococcus aureus and Pseudomonas aeruginosa

Abstract

Keywords

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Encapsulated Phage for Treatment of Burns and Wound Site Infections

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