An electrochemical sensor concept for the detection of virulence factors from Staphylococcus aureus and Pseudomonas aeruginosa

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Abstract

This work presents an electrochemical sensor concept for bacterial toxin detection using biomimetic lipid vesicles containing potassium ferricyanide. The toxin mediated release of redox couples from the vesicles was quantified by measuring the redox current on screen printed electrodes, and the detection limits to rhamnolipid, delta toxin and bacterial supernatants from clinical wound pathogens were examined. Overall detection limits of both redox and carboxyfluorescein containing vesicles were one order of magnitude lower than the cytotoxic dose of studied toxins to T lymphocytes.
LanguageEnglish
Pages104-108
JournalElectrochemistry Communications
Volume59
Early online date9 Jan 2015
DOIs
StatusPublished - Oct 2015

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Electrochemical sensors
Virulence Factors
Bacterial Toxins
T-cells
Biomimetics
Pathogens
Lipids
Potassium
Electrodes
Oxidation-Reduction

Keywords

  • Delta toxin
  • Lipid vesicles
  • Potassium ferricyanide
  • Pseudomonas aeruginosa
  • Rhamnolipid
  • Staphylococcus aureus

Cite this

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title = "An electrochemical sensor concept for the detection of virulence factors from Staphylococcus aureus and Pseudomonas aeruginosa",
abstract = "This work presents an electrochemical sensor concept for bacterial toxin detection using biomimetic lipid vesicles containing potassium ferricyanide. The toxin mediated release of redox couples from the vesicles was quantified by measuring the redox current on screen printed electrodes, and the detection limits to rhamnolipid, delta toxin and bacterial supernatants from clinical wound pathogens were examined. Overall detection limits of both redox and carboxyfluorescein containing vesicles were one order of magnitude lower than the cytotoxic dose of studied toxins to T lymphocytes.",
keywords = "Delta toxin, Lipid vesicles, Potassium ferricyanide, Pseudomonas aeruginosa, Rhamnolipid, Staphylococcus aureus",
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AB - This work presents an electrochemical sensor concept for bacterial toxin detection using biomimetic lipid vesicles containing potassium ferricyanide. The toxin mediated release of redox couples from the vesicles was quantified by measuring the redox current on screen printed electrodes, and the detection limits to rhamnolipid, delta toxin and bacterial supernatants from clinical wound pathogens were examined. Overall detection limits of both redox and carboxyfluorescein containing vesicles were one order of magnitude lower than the cytotoxic dose of studied toxins to T lymphocytes.

KW - Delta toxin

KW - Lipid vesicles

KW - Potassium ferricyanide

KW - Pseudomonas aeruginosa

KW - Rhamnolipid

KW - Staphylococcus aureus

UR - http://www.sciencedirect.com/science/article/pii/S1388248115000028

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T2 - Electrochemistry Communications

JF - Electrochemistry Communications

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