An Analysis of Abdominal-B Expression in the Locust Schistocerca-Gregaria

Robert Kelsh, I Dawson, M Akam

Research output: Contribution to journalArticle

Abstract

We have cloned a homologue of the homeotic gene Abdominal-B from the locust Schistocerca gregaria and raised antibodies against the homeodomain and C-terminal flanking region of the encoded protein. This antibody stains the posterior abdomen of the developing Schistocerca embryo from 26% of development onwards, a stage when only the anterior of the abdomen is visibly segmented. By the time segmentation bas reached the posterior of the abdomen, ABD-B protein is detectable only in the terminal segment (A11). Expression extends anteriorly as development proceeds to include the epidermis of all segments from A8p to A11, including the genital appendages of A9 and A10. The anal cerci, generally regarded as appendages of A11, remain unstained. This expression domain corresponds to the region within which the Abd-B r function of the Drosophila Abd-B gene is expressed. We detect no expression corresponding to the Abd-B m function in Drosophila.
Original languageEnglish
Pages (from-to)293-305
Number of pages13
JournalDevelopment
Volume117
Issue number1
Publication statusPublished - 1993

Fingerprint

Grasshoppers
Abdomen
Drosophila
Antibodies
Homeobox Genes
antineoplaston A10
Epidermis
Embryonic Development
Coloring Agents
Genes
Proteins

Cite this

An Analysis of Abdominal-B Expression in the Locust Schistocerca-Gregaria. / Kelsh, Robert; Dawson, I; Akam, M.

In: Development, Vol. 117, No. 1, 1993, p. 293-305.

Research output: Contribution to journalArticle

Kelsh, R, Dawson, I & Akam, M 1993, 'An Analysis of Abdominal-B Expression in the Locust Schistocerca-Gregaria', Development, vol. 117, no. 1, pp. 293-305.
Kelsh, Robert ; Dawson, I ; Akam, M. / An Analysis of Abdominal-B Expression in the Locust Schistocerca-Gregaria. In: Development. 1993 ; Vol. 117, No. 1. pp. 293-305.
@article{0e8e9356152043809a037b9dc4489a3b,
title = "An Analysis of Abdominal-B Expression in the Locust Schistocerca-Gregaria",
abstract = "We have cloned a homologue of the homeotic gene Abdominal-B from the locust Schistocerca gregaria and raised antibodies against the homeodomain and C-terminal flanking region of the encoded protein. This antibody stains the posterior abdomen of the developing Schistocerca embryo from 26{\%} of development onwards, a stage when only the anterior of the abdomen is visibly segmented. By the time segmentation bas reached the posterior of the abdomen, ABD-B protein is detectable only in the terminal segment (A11). Expression extends anteriorly as development proceeds to include the epidermis of all segments from A8p to A11, including the genital appendages of A9 and A10. The anal cerci, generally regarded as appendages of A11, remain unstained. This expression domain corresponds to the region within which the Abd-B r function of the Drosophila Abd-B gene is expressed. We detect no expression corresponding to the Abd-B m function in Drosophila.",
author = "Robert Kelsh and I Dawson and M Akam",
year = "1993",
language = "English",
volume = "117",
pages = "293--305",
journal = "Development",
issn = "0950-1991",
publisher = "Company of Biologists Ltd",
number = "1",

}

TY - JOUR

T1 - An Analysis of Abdominal-B Expression in the Locust Schistocerca-Gregaria

AU - Kelsh, Robert

AU - Dawson, I

AU - Akam, M

PY - 1993

Y1 - 1993

N2 - We have cloned a homologue of the homeotic gene Abdominal-B from the locust Schistocerca gregaria and raised antibodies against the homeodomain and C-terminal flanking region of the encoded protein. This antibody stains the posterior abdomen of the developing Schistocerca embryo from 26% of development onwards, a stage when only the anterior of the abdomen is visibly segmented. By the time segmentation bas reached the posterior of the abdomen, ABD-B protein is detectable only in the terminal segment (A11). Expression extends anteriorly as development proceeds to include the epidermis of all segments from A8p to A11, including the genital appendages of A9 and A10. The anal cerci, generally regarded as appendages of A11, remain unstained. This expression domain corresponds to the region within which the Abd-B r function of the Drosophila Abd-B gene is expressed. We detect no expression corresponding to the Abd-B m function in Drosophila.

AB - We have cloned a homologue of the homeotic gene Abdominal-B from the locust Schistocerca gregaria and raised antibodies against the homeodomain and C-terminal flanking region of the encoded protein. This antibody stains the posterior abdomen of the developing Schistocerca embryo from 26% of development onwards, a stage when only the anterior of the abdomen is visibly segmented. By the time segmentation bas reached the posterior of the abdomen, ABD-B protein is detectable only in the terminal segment (A11). Expression extends anteriorly as development proceeds to include the epidermis of all segments from A8p to A11, including the genital appendages of A9 and A10. The anal cerci, generally regarded as appendages of A11, remain unstained. This expression domain corresponds to the region within which the Abd-B r function of the Drosophila Abd-B gene is expressed. We detect no expression corresponding to the Abd-B m function in Drosophila.

M3 - Article

VL - 117

SP - 293

EP - 305

JO - Development

JF - Development

SN - 0950-1991

IS - 1

ER -