Amperometric IFN-γ immunosensors with commercially fabricated PCB sensing electrodes

Despina Moschou, Louise Greathead, Panagiotis Pantelidis, Peter Kelleher, Hywel Morgan, Themistoklis Prodromakis

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

Lab-on-a-Chip (LoC) technology has the potential to revolutionize medical Point-of-Care diagnostics. Currently, considerable research efforts are focused on innovative production technologies that will make commercial upscaling of lab-on-chip products financially viable. Printed circuit board (PCB) manufacturing techniques have several advantages in this field. In this paper we focus on transferring a complete IFN-γ enzyme-linked immune-sorbent assay (ELISA) onto a commercial PCB electrochemical biosensing platform, We adapted a commercially available ELISA to detect the enzyme product TMB/H2O2 using amperometry, successfully reproducing the colorimetry-obtained ELISA standard curve. The results demonstrate the potential for the integration of these components into an automated, disposable, electronic ELISA Lab-on-PCB diagnostic platform.

Original languageEnglish
Pages (from-to)805-810
Number of pages6
JournalBiosensors and Bioelectronics
Volume86
Early online date25 Jul 2016
DOIs
Publication statusPublished - 15 Dec 2016

Keywords

  • Amperometric biosensor
  • Immunosensors
  • Lab-on-PCB
  • PCB sensors

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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