Alternative splicing and the evolution of phenotypic novelty

Stephen J. Bush, Lu Chen, Jaime M. Tovar-Corona, Araxi O. Urrutia

Research output: Contribution to journalReview articlepeer-review

113 Citations (SciVal)


Alternative splicing, a mechanism of post-transcriptional RNA processing whereby a single gene can encode multiple distinct transcripts, has been proposed to underlie morphological innovations in multicellular organisms. Genes with developmental functions are enriched for alternative splicing events, suggestive of a contribution of alternative splicing to developmental programmes. The role of alternative splicing as a source of transcript diversification has previously been compared to that of gene duplication, with the relationship between the two extensively explored. Alternative splicing is reduced following gene duplication with the retention of duplicate copies higher for genes whichwere alternatively spliced prior to duplication. Furthermore, and unlike the case for overall gene number, the proportion of alternatively spliced genes has also increased in line with the evolutionary diversification of cell types, suggesting alternative splicing may contribute to the complexity of developmental programmes. Together these observations suggest a prominent role for alternative splicing as a source of functional innovation. However, it is unknown whether the proliferation of alternative splicing events indeed reflects a functional expansion of the transcriptome or instead results from weaker selection acting on larger species, which tend to have a higher number of cell types and lower population sizes.

Original languageEnglish
Article number20150474
JournalProceedings of the Royal Society B: Biological Sciences
Issue number1713
Publication statusPublished - 5 Feb 2017


  • Alternative splicing
  • Comparative genomics
  • Effective population size
  • Functional innovation
  • Gene duplication
  • Genetic drift


Dive into the research topics of 'Alternative splicing and the evolution of phenotypic novelty'. Together they form a unique fingerprint.

Cite this