Alpha-synuclein ferrireductase activity is detectible in vivo, is altered in Parkinson's disease and increases the neurotoxicity of DOPAL

Jennifer S. McDowall, Ioanna Ntai, Kevin C. Honeychurch, John P. Hart, Philippe Colin, Bernard L. Schneider, David Brown

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)
157 Downloads (Pure)

Abstract

The normal cellular role of α-synuclein is of potential importance in understanding diseases in which an aggregated form of the protein has been implicated. A potential loss or change in the normal function of α-synuclein could play a role in the aetiology of diseases such as Parkinson's disease. Recently, it has been suggested that α-synuclein could cause the enzymatic reduction of iron and a cellular increase in Fe(II) levels. Experiments were carried out to determine if such activity could be measured in vivo. Experiments with rats overexpressing human α-synuclein in nigral dopaminergic neurons demonstrated a correlation between α-synuclein expression and ferrireductase activity. Furthermore, studies on tissue from Parkinson's disease patient brains showed a significant decrease in ferrireductase activity, possibly due to deposition of large amounts of inactive protein. Cellular studies suggest that increase ferrireductase activity results in increased levels of dopamine metabolites and increased sensitivity to the toxicity of DOPAL. These findings demonstrate that α-synuclein ferrireductase activity is present in vivo and its alteration may play a role in neuron loss in disease.

Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalMolecular and Cellular Neuroscience
Volume85
Early online date12 Aug 2017
DOIs
Publication statusPublished - 1 Dec 2017

Keywords

  • DOPAL
  • Ferrireductase
  • Iron
  • Neurotoxicity
  • Synuclein

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology

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