Allozyme analysis of Haemonchus contortus resistant and susceptible to anthelmintics, with an indication of dipeptidases associated with resistance

V. Šnábel, A. Königová, M. Dolinská, M. Várady, A.J. Wolstenholme

Research output: Contribution to journalArticle

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Abstract

Summary: In an attempt to investigate the genetic and biochemical causes underlying resistance to anthelmintics, the trichostrongylid nematode Haemonchus contortus was examined by an allozymic analysis of nine candidate geneenzyme systems. Two strains resistant to benzimidazoles, two multidrug-resistant strains, and three strains susceptible to anthelmintics isolated from three continents were examined by isoelectric focusing. Two enzymes (mannose phosphate isomerase and peptidase-D) had polymorphic patterns. The peptidase-D zymograms indicated an association with the selection for resistance. Susceptible isolates gave more complex patterns due to the formation of a heterodimer controlled by the two active loci, whereas in resistant isolates, only a single locus with one electromorph was responsible for the profiles. A possible link between dipeptidases and the acquisition of resistance through the turnover of proteinrich collagen, and changes in membrane-associated genes that influence drug efflux, are discussed.
Original languageEnglish
Pages (from-to)128-133
Number of pages6
JournalHelminthologia
Volume49
Issue number3
DOIs
Publication statusPublished - 1 Sep 2012

Fingerprint

proline dipeptidase
Dipeptidases
dipeptidases
Haemonchus
Anthelmintics
Haemonchus contortus
anthelmintics
allozymes
Isoenzymes
Benzimidazoles
mannose-6-phosphate isomerase
Isoelectric Focusing
benzimidazoles
Trichostrongylidae
Molecular Biology
loci
Collagen
Phosphates
isoelectric focusing
Membranes

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Allozyme analysis of Haemonchus contortus resistant and susceptible to anthelmintics, with an indication of dipeptidases associated with resistance. / Šnábel, V.; Königová, A.; Dolinská, M.; Várady, M.; Wolstenholme, A.J.

In: Helminthologia, Vol. 49, No. 3, 01.09.2012, p. 128-133.

Research output: Contribution to journalArticle

Šnábel, V. ; Königová, A. ; Dolinská, M. ; Várady, M. ; Wolstenholme, A.J. / Allozyme analysis of Haemonchus contortus resistant and susceptible to anthelmintics, with an indication of dipeptidases associated with resistance. In: Helminthologia. 2012 ; Vol. 49, No. 3. pp. 128-133.
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