Allelic variation in the Arabidopsis TNL CHS3/CSA1 immune receptor pair reveals two functional cell-death regulatory modes

Yu Yang, Nak Hyun Kim, Volkan Cevik, Pierre Jacob, Li Wan, Oliver J. Furzer, Jeffery L. Dangl

Research output: Contribution to journalArticlepeer-review

Abstract

Some plant NLR immune receptors are encoded in head-to-head “sensor-executor” pairs that function together. Alleles of the NLR pair CHS3/CSA1 form three clades. The clade 1 sensor CHS3 contains an integrated domain (ID) with homology to regulatory domains, which is lacking in clades 2 and 3. In this study, we defined two cell-death regulatory modes for CHS3/CSA1 pairs. One is mediated by ID domain on clade 1 CHS3, and the other relies on CHS3/CSA1 pairs from all clades detecting perturbation of an associated pattern-recognition receptor (PRR) co-receptor. Our data support the hypothesis that an ancestral Arabidopsis CHS3/CSA1 pair gained a second recognition specificity and regulatory mechanism through ID acquisition while retaining its original specificity as a “guard” against PRR co-receptor perturbation. This likely comes with a cost, since both ID and non-ID alleles of the pair persist in diverse Arabidopsis populations through balancing selection.

Original languageEnglish
Pages (from-to)1701-1716.e5
JournalCell Host and Microbe
Volume30
Issue number12
Early online date17 Oct 2022
DOIs
Publication statusPublished - 14 Dec 2022

Keywords

  • BAK1
  • CHS3
  • co-evolution
  • CSA1
  • guard hypothesis
  • paired NLR
  • plant immune system
  • specialization

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Virology

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