Abstract
Some plant NLR immune receptors are encoded in head-to-head “sensor-executor” pairs that function together. Alleles of the NLR pair CHS3/CSA1 form three clades. The clade 1 sensor CHS3 contains an integrated domain (ID) with homology to regulatory domains, which is lacking in clades 2 and 3. In this study, we defined two cell-death regulatory modes for CHS3/CSA1 pairs. One is mediated by ID domain on clade 1 CHS3, and the other relies on CHS3/CSA1 pairs from all clades detecting perturbation of an associated pattern-recognition receptor (PRR) co-receptor. Our data support the hypothesis that an ancestral Arabidopsis CHS3/CSA1 pair gained a second recognition specificity and regulatory mechanism through ID acquisition while retaining its original specificity as a “guard” against PRR co-receptor perturbation. This likely comes with a cost, since both ID and non-ID alleles of the pair persist in diverse Arabidopsis populations through balancing selection.
| Original language | English |
|---|---|
| Pages (from-to) | 1701-1716.e5 |
| Journal | Cell Host and Microbe |
| Volume | 30 |
| Issue number | 12 |
| Early online date | 17 Oct 2022 |
| DOIs | |
| Publication status | Published - 14 Dec 2022 |
Bibliographical note
Funding Information:We thank Sarah Grant, Farid El-Kasmi, and Marc T. Nishimura for excellent suggestions and comments and Jia Li for providing the bak1-3 bkk1-1 seeds. Supported by National Science Foundation (grant IOS-1758400 to J.L.D.) and HHMI . J.L.D. is a Howard Hughes Medical Institute (HHMI) Investigator. N.H.K. was partially supported by Basic Science Research Program through the National Research Foundation of Korea Fellowship funded by the Ministry of Education ( 2014R1A6A3A03058629 ). This article is subject to HHMI’s Open Access to Publications policy. HHMI lab heads have previously granted a nonexclusive CC BY 4.0 license to the public and a sublicensable license to HHMI in their research articles. Pursuant to those licenses, the author-accepted manuscript of this article can be made freely available under a CC BY4.0 license immediately upon publication.
Funding
We thank Sarah Grant, Farid El-Kasmi, and Marc T. Nishimura for excellent suggestions and comments and Jia Li for providing the bak1-3 bkk1-1 seeds. Supported by National Science Foundation (grant IOS-1758400 to J.L.D.) and HHMI . J.L.D. is a Howard Hughes Medical Institute (HHMI) Investigator. N.H.K. was partially supported by Basic Science Research Program through the National Research Foundation of Korea Fellowship funded by the Ministry of Education ( 2014R1A6A3A03058629 ). This article is subject to HHMI’s Open Access to Publications policy. HHMI lab heads have previously granted a nonexclusive CC BY 4.0 license to the public and a sublicensable license to HHMI in their research articles. Pursuant to those licenses, the author-accepted manuscript of this article can be made freely available under a CC BY4.0 license immediately upon publication.
Keywords
- BAK1
- CHS3
- co-evolution
- CSA1
- guard hypothesis
- paired NLR
- plant immune system
- specialization
ASJC Scopus subject areas
- Parasitology
- Microbiology
- Virology