Albumin-derived nanocarriers

Substrates for enhanced cell adhesive ligand display and cell motility

Ram I Sharma, Marian Pereira, Jean E Schwarzbauer, Prabhas V Moghe

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Cell-adhesive ligands organized on nanoscale synthetic biomaterials can potentially recapitulate the nanoscale organization of extracellular matrix and the consequent effects of cell dynamics. In this study, 100 nm albumin nanocarriers (ANC) were fabricated to serve as nanoscale organizational units for a well-defined ligand, the recombinant fragment from fibronectin comprised of the RGD-containing module 10 and the synergy-region-containing module 9. Conventional protein conjugation chemistry was employed to fabricate nanocarriers with increasing levels of displayed ligand. Presentation of ligand-functionalized ANCs adsorbed onto substrates was found to enhance keratinocyte attachment when compared to equivalent levels of adsorbed ligands, supported by ELISA data that the display of ligand on ANCs essentially increased the accessibility of the cell-binding domain and AFM data that the ligand was likely exposed due to ligand-ANC repulsion. The ligand presentation from ANCs converted the cellular morphology from a stationary phenotype to a motile phenotype, with the expression of filopodia-like microextensions, and a decrease in focal adhesions, indicating decreased cell adhesion strength. Consequently, cell motility was found to be significantly elevated on ligand-ANC substrates relative to substrates with equivalent levels of ligand. Overall, the ligand-functionalized albumin nanocarriers offer a unique model platform with two distinct properties: enhanced ligand exposure for enhancement of cell attachment to ligands at low concentrations; and enhanced cell detachment, motile phenotype, and migration kinetics.
Original languageEnglish
Pages (from-to)3589-3598
Number of pages10
JournalBiomaterials
Volume27
Issue number19
DOIs
Publication statusPublished - 2006

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Adhesives
Cell Movement
Albumins
Display devices
Ligands
Substrates
Phenotype
Data Display
Pseudopodia
Focal Adhesions
Bond strength (materials)
Cell adhesion
Biocompatible Materials
Keratinocytes
Fibronectins
Biomaterials
Cell Adhesion
Extracellular Matrix
Adhesion
Enzyme-Linked Immunosorbent Assay

Cite this

Albumin-derived nanocarriers : Substrates for enhanced cell adhesive ligand display and cell motility. / Sharma, Ram I; Pereira, Marian; Schwarzbauer, Jean E; Moghe, Prabhas V.

In: Biomaterials, Vol. 27, No. 19, 2006, p. 3589-3598.

Research output: Contribution to journalArticle

Sharma, Ram I ; Pereira, Marian ; Schwarzbauer, Jean E ; Moghe, Prabhas V. / Albumin-derived nanocarriers : Substrates for enhanced cell adhesive ligand display and cell motility. In: Biomaterials. 2006 ; Vol. 27, No. 19. pp. 3589-3598.
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