In recent years, there has been an increasing concern about non-governmental development organisations’ (NGDOs) sustainability especially in countries including Ghana that have transitioned into lower-middle-income status. The effect has been donor withdrawal and funding cuts for NGDOs. This presents opportunities and challenges for NGDOs in their attempt to mobilise alternative funding routes in ensuring their sustainability. Drawing on secondary literature and semi-structured interviews with fifty-seven respondents from national NGDOs, government, donors and corporate organisations, this article documents and expands our understanding of the different typologies of philanthropic institutions in Ghana as potential alternative funding routes for NGDOs. It finds that a weak enabling environment including the absence of a regulatory framework and fiscal incentives for domestic resource mobilisation stands to affect the potential of philanthropic institutions as alternative funding routes for NGDOs’ sustainability.