Agroinfiltration of Nicotiana benthamiana leaves for co-localization of regulatory proteins involved in jasmonate signaling

Volkan Cevik, Kemal Kazan

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

Protein-protein interactions play important roles in many cellular processes, including the regulation of phytohormone signaling pathways. Identification of interacting partners of key proteins involved in the cellular signaling control can provide potentially unexpected insights into the molecular events occurring in any signaling pathway. Over the years, various techniques have been developed to examine protein-protein interactions, but, besides certain advantages, most of them have various pitfalls, such as yielding nonspecific interactions. Therefore, additional information obtained through different methods may be needed to substantiate protein-protein interaction data. One of these techniques involves the co-localization of proteins suspected to interact in the same subcellular compartment. In this chapter, we describe a method for co-expression of proteins associated with jasmonate signaling in Nicotiana benthamiana for studies such as co-localization.

Original languageEnglish
Title of host publicationJasmonate Signaling
Subtitle of host publicationMethods and Protocols, Part III
EditorsA. Goossens , L. Pauwels
PublisherHumana Press
Pages199-208
Number of pages10
ISBN (Print)9781627034135
DOIs
Publication statusPublished - Apr 2013

Publication series

NameMethods in Molecular Biology : Methods and Protocols
Volume1011

Keywords

  • Agrobacterium
  • Culture Techniques
  • Escherichia coli
  • Intracellular Signaling Peptides and Proteins
  • Luminescent Proteins
  • Microscopy, Confocal
  • Microscopy, Fluorescence
  • Plant Epidermis
  • Plant Leaves
  • Plant Proteins
  • Protein Interaction Mapping
  • Protein Transport
  • Signal Transduction
  • Tobacco
  • Transformation, Bacterial
  • Journal Article

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  • Cite this

    Cevik, V., & Kazan, K. (2013). Agroinfiltration of Nicotiana benthamiana leaves for co-localization of regulatory proteins involved in jasmonate signaling. In A. Goossens , & L. Pauwels (Eds.), Jasmonate Signaling: Methods and Protocols, Part III (pp. 199-208). (Methods in Molecular Biology : Methods and Protocols; Vol. 1011). Humana Press. https://doi.org/10.1007/978-1-62703-414-2_16