Ageing: cloning of mice to six generations

T Wakayama; Y Shinkai; K L K Tamashiro; H Niida; D C Blanchard; R J Blanchard; A Ogura; K Tanemura; M Tachibana; Anthony C F Perry; D F Colgan; P Mombaerts; R Yanagimachi

doi:10.1038/35030301

Ageing: cloning of mice to six generations

T Wakayama, Y Shinkai, K L K Tamashiro, H Niida, D C Blanchard, R J Blanchard, A Ogura, K Tanemura, M Tachibana, Anthony C F Perry, D F Colgan, P Mombaerts, R Yanagimachi

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

212 Citations (SciVal)

Abstract

Mice have been cloned by nuclear transfer into enucleated oocytes and here we describe the reiterative cloning of mice to four and six generations in two independent lines. Successive generations showed no signs of prematureageing, as judged by gross behavioural parameters, and there was no evidence of shortening of telomeres at the ends of chromosomes, normally an indicator of cellular senescence — in fact, these appeared to increase slightly in length. This increase is surprising, given that the number of mitotic divisions greatly exceeds that of sexually produced animals and that any deleterious effects of cloning might be expected to be amplified in sequentially cloned mice. Our results offer a new approach to the study of organismal ageing.

Original language	English
Pages (from-to)	318-319
Number of pages	2
Journal	Nature
Volume	407
Issue number	6802
DOIs	https://doi.org/10.1038/35030301
Publication status	Published - 2000

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title = "Ageing: cloning of mice to six generations",

abstract = "Mice have been cloned by nuclear transfer into enucleated oocytes and here we describe the reiterative cloning of mice to four and six generations in two independent lines. Successive generations showed no signs of prematureageing, as judged by gross behavioural parameters, and there was no evidence of shortening of telomeres at the ends of chromosomes, normally an indicator of cellular senescence — in fact, these appeared to increase slightly in length. This increase is surprising, given that the number of mitotic divisions greatly exceeds that of sexually produced animals and that any deleterious effects of cloning might be expected to be amplified in sequentially cloned mice. Our results offer a new approach to the study of organismal ageing.",

author = "T Wakayama and Y Shinkai and Tamashiro, {K L K} and H Niida and Blanchard, {D C} and Blanchard, {R J} and A Ogura and K Tanemura and M Tachibana and Perry, {Anthony C F} and Colgan, {D F} and P Mombaerts and R Yanagimachi",

year = "2000",

doi = "10.1038/35030301",

language = "English",

volume = "407",

pages = "318--319",

journal = "Nature",

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TY - JOUR

T1 - Ageing: cloning of mice to six generations

AU - Wakayama, T

AU - Shinkai, Y

AU - Tamashiro, K L K

AU - Niida, H

AU - Blanchard, D C

AU - Blanchard, R J

AU - Ogura, A

AU - Tanemura, K

AU - Tachibana, M

AU - Perry, Anthony C F

AU - Colgan, D F

AU - Mombaerts, P

AU - Yanagimachi, R

PY - 2000

Y1 - 2000

N2 - Mice have been cloned by nuclear transfer into enucleated oocytes and here we describe the reiterative cloning of mice to four and six generations in two independent lines. Successive generations showed no signs of prematureageing, as judged by gross behavioural parameters, and there was no evidence of shortening of telomeres at the ends of chromosomes, normally an indicator of cellular senescence — in fact, these appeared to increase slightly in length. This increase is surprising, given that the number of mitotic divisions greatly exceeds that of sexually produced animals and that any deleterious effects of cloning might be expected to be amplified in sequentially cloned mice. Our results offer a new approach to the study of organismal ageing.

AB - Mice have been cloned by nuclear transfer into enucleated oocytes and here we describe the reiterative cloning of mice to four and six generations in two independent lines. Successive generations showed no signs of prematureageing, as judged by gross behavioural parameters, and there was no evidence of shortening of telomeres at the ends of chromosomes, normally an indicator of cellular senescence — in fact, these appeared to increase slightly in length. This increase is surprising, given that the number of mitotic divisions greatly exceeds that of sexually produced animals and that any deleterious effects of cloning might be expected to be amplified in sequentially cloned mice. Our results offer a new approach to the study of organismal ageing.

UR - http://dx.doi.org/10.1038/35030301

U2 - 10.1038/35030301

DO - 10.1038/35030301

M3 - Article

SN - 0028-0836

VL - 407

SP - 318

EP - 319

JO - Nature

JF - Nature

IS - 6802

ER -

Ageing: cloning of mice to six generations

Abstract

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