TY - JOUR
T1 - Additive manufactured graphene-based electrodes exhibit beneficial performances in Pseudomonas aeruginosa microbial fuel cells
AU - Slate, Anthony J.
AU - Hickey, Niall A.
AU - Butler, Jonathan A.
AU - Wilson, Daniel
AU - Liauw, Christopher M.
AU - Banks, Craig E.
AU - Whitehead, Kathryn A.
N1 - Funding Information:
The Manchester Fuel Cell Innovation Centre is funded by the European Regional Development Fund.
Funding Information:
The authors wish to acknowledge technical support provided by Hayley Andrews (Manchester Metropolitan University, UK) for assistance with Scanning Electron Microscopy.
Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2021/7/1
Y1 - 2021/7/1
N2 - A commercial polylactic acid/graphene (8 wt%) composite filament was used to additive manufacture (AM) graphene macroelectrodes (AM-GMs). The electrode surfaces were characterised and Pseudomonas aeruginosa was utilised as the exoelectrogen. The MFC was optimised using growth kinetic assays, biofilm formation, and quantification of pyocyanin production (via liquid chromatography-mass spectrometry) in conditions that were representative of the batch-fed MFC configuration utilised. Cell potential and bacterial viability was recorded at 0 h, 24 h, 48 h, 72 h, 96 h and 120 h, power density and current density were calculated. There was no significant difference between P. aeruginosa cell proliferation in either media tested. Interestingly, no accumulation of pyocyanin was evident. Additively manufactured electrodes comprised of graphene (AM-GMs) were successfully applied in a P. aeruginosa MFC configuration and power outputs (110.74 ± 14.63 μW m-2) produced were comparable to that of the ‘benchmark’ electrode, carbon cloth (93.49 ± 5.17 μW m-2). The AM-GMs demonstrated power/current outputs similar to that of the carbon cloth electrodes in both anaerobic LB and glucose-based media over 120 h; the AM-GMs had no significant detrimental effect on P. aeruginosa viability. This study highlights the potential application of additive manufactured electrodes with the incorporation of nanomaterials (e.g., graphene) as one approach to enhance power outputs.
AB - A commercial polylactic acid/graphene (8 wt%) composite filament was used to additive manufacture (AM) graphene macroelectrodes (AM-GMs). The electrode surfaces were characterised and Pseudomonas aeruginosa was utilised as the exoelectrogen. The MFC was optimised using growth kinetic assays, biofilm formation, and quantification of pyocyanin production (via liquid chromatography-mass spectrometry) in conditions that were representative of the batch-fed MFC configuration utilised. Cell potential and bacterial viability was recorded at 0 h, 24 h, 48 h, 72 h, 96 h and 120 h, power density and current density were calculated. There was no significant difference between P. aeruginosa cell proliferation in either media tested. Interestingly, no accumulation of pyocyanin was evident. Additively manufactured electrodes comprised of graphene (AM-GMs) were successfully applied in a P. aeruginosa MFC configuration and power outputs (110.74 ± 14.63 μW m-2) produced were comparable to that of the ‘benchmark’ electrode, carbon cloth (93.49 ± 5.17 μW m-2). The AM-GMs demonstrated power/current outputs similar to that of the carbon cloth electrodes in both anaerobic LB and glucose-based media over 120 h; the AM-GMs had no significant detrimental effect on P. aeruginosa viability. This study highlights the potential application of additive manufactured electrodes with the incorporation of nanomaterials (e.g., graphene) as one approach to enhance power outputs.
KW - Additive manufacturing
KW - Biotechnology
KW - Graphene
KW - Microbial fuel cell
KW - Pseudomonas aeruginosa
KW - Pyocyanin
UR - http://www.scopus.com/inward/record.url?scp=85105299243&partnerID=8YFLogxK
U2 - 10.1016/j.jpowsour.2021.229938
DO - 10.1016/j.jpowsour.2021.229938
M3 - Article
AN - SCOPUS:85105299243
SN - 0378-7753
VL - 499
JO - Journal of Power Sources
JF - Journal of Power Sources
M1 - 229938
ER -
