Adaxial cell migration in the zebrafish embryo is an active cell autonomous property that requires the Prdm1a transcription factor

Yosuke Ono, Weimiao Yu, Harriet E Jackson, Caroline A Parkin, Philip W Ingham

Research output: Contribution to journalArticlepeer-review

8 Citations (SciVal)

Abstract

Adaxial cells, the progenitors of slow-twitch muscle fibres in zebrafish, exhibit a stereotypic migratory behaviour during somitogenesis. Although this process is known to be disrupted in various mutants, its precise nature has remained unclear. Here, using in vivo imaging and chimera analysis, we show that adaxial cell migration is a cell autonomous process, during which cells become polarised and extend filopodia at their leading edge. Loss of function of the Prdm1a transcription factor disrupts the polarisation and migration of adaxial cells, reflecting a role that is independent of its repression of sox6 expression. Expression of the M- and N-cadherins, previously implicated in driving adaxial cell migration, is largely unaffected by loss of Prdm1a function, suggesting that differential cadherin expression is not sufficient for adaxial cell migration.

Original languageEnglish
Pages (from-to)77-86
Number of pages10
JournalDifferentiation
Volume89
Issue number3-4
DOIs
Publication statusPublished - 7 Apr 2015

Bibliographical note

Copyright © 2015 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.

Keywords

  • Animals
  • Cadherins/biosynthesis
  • Cell Differentiation/genetics
  • Cell Movement/genetics
  • DNA-Binding Proteins/biosynthesis
  • Embryonic Development/genetics
  • Gene Expression Regulation, Developmental
  • Muscle, Skeletal/growth & development
  • Nuclear Proteins/biosynthesis
  • Positive Regulatory Domain I-Binding Factor 1
  • Zebrafish/genetics
  • Zebrafish Proteins/biosynthesis

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