Active screen plasma nitriding enhances cell attachment to polymer surfaces

G Kaklamani, J Bowen, N Mehrban, H Dong, LM Grover, A Stamboulis

Research output: Contribution to journalArticlepeer-review

25 Citations (SciVal)

Abstract

Active screen plasma nitriding (ASPN) is a well-established technique used for the surface modification of materials, the result of which is often a product with enhanced functional performance. Here we report the modification of the chemical and mechanical properties of ultra-high molecular weight poly(ethylene) (UHMWPE) using 80:20 (v/v) N2/H2 ASPN, followed by growth of 3T3 fibroblasts on the treated and untreated polymer surfaces. ASPN-treated UHMWPE showed extensive fibroblast attachment within 3 h of seeding, whereas fibroblasts did not successfully attach to untreated UHMWPE. Fibroblast-coated surfaces were maintained for up to 28 days, monitoring their metabolic activity and morphology throughout. The chemical properties of the ASPN-treated UHMWPE surface were studied using X-ray photoelectron spectroscopy, revealing the presence of Csingle bondN, Cdouble bondN, and Ctriple bondN chemical bonds. The elastic modulus, surface topography, and adhesion properties of the ASPN-treated UHMWPE surface were studied over 28 days during sample storage under ambient conditions and during immersion in two commonly used cell culture media.

Original languageEnglish
Pages (from-to)787-798
JournalApplied Surface Science
Volume273
DOIs
Publication statusPublished - 15 May 2013
Externally publishedYes

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