ACR-26: A novel nicotinic receptor subunit of parasitic nematodes

Hayley M Bennett, Sally M Williamson, T K Walsh, D J Woods, Adrian J Wolstenholme

Research output: Contribution to journalArticle

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Abstract

Nematode nicotinic acetylcholine receptors are the targets for many effective anthelmintics, including those recently introduced into the market. We have identified a novel nicotinic receptor subunit sequence, acr-26, that is expressed in all the animal parasitic nematodes we examined from clades III, IV and V, but is not present in the genomes of Trichinella spiralis, Caenorhabditis elegans, Pristionchus pacificus and Meloidogyne spp. In Ascaris suum, ACR-26 is expressed on muscle cells isolated from the head, but not from the mid-body region. Sequence comparisons with other vertebrate and nematode subunits suggested that ACR-26 may be capable of forming a functional homomeric receptor; when acr-26 cRNA was injected into Xenopus oocytes along with Xenopus laevis ric-3 cRNA we occasionally observed the formation of acetylcholine- and nicotine-sensitive channels. The unreliable expression of ACR-26 in vitro may suggest that additional subunits or chaperones may be required for efficient formation of the functional receptors. ACR-26 may represent a novel target for the development of cholinergic anthelmintics specific for animal parasites.
LanguageEnglish
Pages151-157
Number of pages7
JournalMolecular and Biochemical Parasitology
Volume183
Issue number2
DOIs
StatusPublished - Jun 2012

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Complementary RNA
Anthelmintics
Nicotinic Receptors
Tylenchoidea
Ascaris suum
Trichinella spiralis
Body Regions
Caenorhabditis elegans
Xenopus laevis
Xenopus
Nicotine
Muscle Cells
Cholinergic Agents
Acetylcholine
Oocytes
Vertebrates
Parasites
Head
Genome
In Vitro Techniques

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Bennett, H. M., Williamson, S. M., Walsh, T. K., Woods, D. J., & Wolstenholme, A. J. (2012). ACR-26: A novel nicotinic receptor subunit of parasitic nematodes. DOI: 10.1016/j.molbiopara.2012.02.010

ACR-26: A novel nicotinic receptor subunit of parasitic nematodes. / Bennett, Hayley M; Williamson, Sally M; Walsh, T K; Woods, D J; Wolstenholme, Adrian J.

In: Molecular and Biochemical Parasitology, Vol. 183, No. 2, 06.2012, p. 151-157.

Research output: Contribution to journalArticle

Bennett, HM, Williamson, SM, Walsh, TK, Woods, DJ & Wolstenholme, AJ 2012, 'ACR-26: A novel nicotinic receptor subunit of parasitic nematodes' Molecular and Biochemical Parasitology, vol. 183, no. 2, pp. 151-157. DOI: 10.1016/j.molbiopara.2012.02.010
Bennett HM, Williamson SM, Walsh TK, Woods DJ, Wolstenholme AJ. ACR-26: A novel nicotinic receptor subunit of parasitic nematodes. Molecular and Biochemical Parasitology. 2012 Jun;183(2):151-157. Available from, DOI: 10.1016/j.molbiopara.2012.02.010
Bennett, Hayley M ; Williamson, Sally M ; Walsh, T K ; Woods, D J ; Wolstenholme, Adrian J. / ACR-26: A novel nicotinic receptor subunit of parasitic nematodes. In: Molecular and Biochemical Parasitology. 2012 ; Vol. 183, No. 2. pp. 151-157
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