A thin film detection/response system for pathogenic bacteria

Jin Zhou, Andrew L Loftus, Geraldine Mulley, A Toby A Jenkins

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

This paper describes the modification of nonwoven fabric such that it responds by releasing an encapsulated antimicrobial from within an attached vesicle in response to two species of pathogenic bacteria (Staphylococcus aureus MSSA 476 and Pseudomonas aeruginosa PAO1), but does not respond to nonpathogenic Escherichia coli DH5 alpha. This concept is based on the generalization that a majority of pathogenic bacteria secrete virulence factors such as toxins and lipases that actively damage cell membranes, typically observed as tissue damage around infected wounds, while nonpathogenic bacteria do not (or not at high concentration). The eventual aim of this work is to produce responsive dressings which release antimicrobials and change color only on infected wounds. This paper details preliminary approaches to achieving this goal, including vesicle bacteria studies in aqueous suspension, and fluorescence imaging of fluorescein containing vesicles lysed by S. aureus and P. aeruginosa, but not by E. coli.
Original languageEnglish
Pages (from-to)6566-6570
Number of pages5
JournalJournal of the American Chemical Society
Volume132
Issue number18
Early online date19 Apr 2010
DOIs
Publication statusPublished - 12 May 2010

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