A thermostable aldolase for the synthesis of 3-deoxy-2-ulosonic acids

H J Lamble; Sylvain F Royer; David W Hough; Michael J Danson; G L Taylor; S D Bull

doi:10.1002/adsc.200600520

A thermostable aldolase for the synthesis of 3-deoxy-2-ulosonic acids

H J Lamble
, Sylvain F Royer
, David W Hough
, Michael J Danson
, G L Taylor
, S D Bull

Research output: Contribution to journal › Article › peer-review

17 Citations (SciVal)

Abstract

A stereochemically promiscuous 2-keto3-deoxygluconate aldolase has been used as an efficient biocatalyst to catalyse the aldol reaction of pyruvate with C-3- and C-4-aldoses to afford syn- and anti-3-deoxy-2-ulosonic acids in poor to good de. A continuous flow bioreactor containing immobilised aldolase has been developed that enables gram quantities of C-6- and C-7-3-deoxyhept-2-ulosonic acids to be produced in an efficient manner.

Original language	English
Pages (from-to)	817-821
Number of pages	5
Journal	Advanced Synthesis & Catalysis
Volume	349
Issue number	6
DOIs	https://doi.org/10.1002/adsc.200600520
Publication status	Published - 2007

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ID number: ISI:000246040000006

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10.1002/adsc.200600520

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title = "A thermostable aldolase for the synthesis of 3-deoxy-2-ulosonic acids",

abstract = "A stereochemically promiscuous 2-keto3-deoxygluconate aldolase has been used as an efficient biocatalyst to catalyse the aldol reaction of pyruvate with C-3- and C-4-aldoses to afford syn- and anti-3-deoxy-2-ulosonic acids in poor to good de. A continuous flow bioreactor containing immobilised aldolase has been developed that enables gram quantities of C-6- and C-7-3-deoxyhept-2-ulosonic acids to be produced in an efficient manner.",

author = "Lamble, \{H J\} and Royer, \{Sylvain F\} and Hough, \{David W\} and Danson, \{Michael J\} and Taylor, \{G L\} and Bull, \{S D\}",

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language = "English",

volume = "349",

pages = "817--821",

journal = "Advanced Synthesis \& Catalysis",

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T1 - A thermostable aldolase for the synthesis of 3-deoxy-2-ulosonic acids

AU - Lamble, H J

AU - Royer, Sylvain F

AU - Hough, David W

AU - Danson, Michael J

AU - Taylor, G L

AU - Bull, S D

N1 - ID number: ISI:000246040000006

PY - 2007

Y1 - 2007

N2 - A stereochemically promiscuous 2-keto3-deoxygluconate aldolase has been used as an efficient biocatalyst to catalyse the aldol reaction of pyruvate with C-3- and C-4-aldoses to afford syn- and anti-3-deoxy-2-ulosonic acids in poor to good de. A continuous flow bioreactor containing immobilised aldolase has been developed that enables gram quantities of C-6- and C-7-3-deoxyhept-2-ulosonic acids to be produced in an efficient manner.

AB - A stereochemically promiscuous 2-keto3-deoxygluconate aldolase has been used as an efficient biocatalyst to catalyse the aldol reaction of pyruvate with C-3- and C-4-aldoses to afford syn- and anti-3-deoxy-2-ulosonic acids in poor to good de. A continuous flow bioreactor containing immobilised aldolase has been developed that enables gram quantities of C-6- and C-7-3-deoxyhept-2-ulosonic acids to be produced in an efficient manner.

UR - http://dx.doi.org/10.1002/adsc.200600520

U2 - 10.1002/adsc.200600520

DO - 10.1002/adsc.200600520

M3 - Article

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VL - 349

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EP - 821

JO - Advanced Synthesis & Catalysis

JF - Advanced Synthesis & Catalysis

IS - 6

ER -

A thermostable aldolase for the synthesis of 3-deoxy-2-ulosonic acids

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METABOLIC PATHWAY PROMISCUITY: A STRUCTURAL, MECHANISTIC AN D BIOCATALYTIC INVESTIGATION

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A thermostable aldolase for the synthesis of 3-deoxy-2-ulosonic acids

Abstract

Bibliographical note

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METABOLIC PATHWAY PROMISCUITY: A STRUCTURAL, MECHANISTIC AN D BIOCATALYTIC INVESTIGATION

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