A stereochemically promiscuous 2-keto3-deoxygluconate aldolase has been used as an efficient biocatalyst to catalyse the aldol reaction of pyruvate with C-3- and C-4-aldoses to afford syn- and anti-3-deoxy-2-ulosonic acids in poor to good de. A continuous flow bioreactor containing immobilised aldolase has been developed that enables gram quantities of C-6- and C-7-3-deoxyhept-2-ulosonic acids to be produced in an efficient manner.
Lamble, H. J., Royer, S. F., Hough, D. W., Danson, M. J., Taylor, G. L., & Bull, S. D. (2007). A thermostable aldolase for the synthesis of 3-deoxy-2-ulosonic acids. Advanced Synthesis & Catalysis, 349(6), 817-821. https://doi.org/10.1002/adsc.200600520