A temperature compensation method for chlorophyll and phycocyanin fluorescence sensors in freshwater

C. J. Watras, K. A. Morrison, J. L. Rubsam, P. C. Hanson, A. J. Watras, G. D. Laliberte, P. Milewski

Research output: Contribution to journalArticle

Abstract

The in vivo fluorescence (IVF) of photosynthetic pigments is used widely as a proxy for phytoplankton biomass in fresh and marine waters. Although fluorescence intensity is known to decrease with rising temperature for many fluorophores, temperature quench is rarely accounted for in field studies of plankton IVF. Here, we quantified the effect of temperature on in vivo chlorophyll and phycocyanin fluorescence in the laboratory (∼ 5°C to 30°C), and we derived temperature compensation equations for IVF sensors commonly used in freshwaters. The equations reference measured fluorescence to a standard temperature, and they have the same linear form as the equation derived in an earlier study of chromophoric dissolved organic matter fluorescence: Fr=Fm/(1+ρ(Tm - Tr)), where F is fluorescence intensity (RFU, relative fluorescence units), T is temperature (°C), ρ is the temperature coefficient at a given reference temperature (°C-1), and the subscripts r and m stand for the reference and measured values. At a reference temperature of 20°C, the temperature coefficients (ρ) for chlorophyll and phycocyanin in Wisconsin lake waters ranged from -0.008°C-1 to -0.012°C-1 and -0.006°C-1 to -0.012°C-1, respectively. For chlorophyll in a pure culture of the green alga Scenedesmus dimorphus, the value for ρ was similar to the value in natural assemblages, averaging -0.018±0.003°C-1; but for phycocyanin in the blue-green alga Synechococcus leopoliensis it was lower (more negative), averaging -0.034±0.003°C-1. This disparity notwithstanding, we conclude that temperature compensation is an important component of IVF monitoring.

LanguageEnglish
Pages642-652
JournalLimnology and Oceanography: Methods
Volume15
Issue number7
Early online date13 May 2017
DOIs
StatusPublished - Jul 2017

Fingerprint

Chlorophyll
Fluorescence
Sensors
Temperature
Compensation and Redress
Plankton
Fluorophores
Phytoplankton
Algae
Pigments
Biological materials
Lakes
Water
Biomass

ASJC Scopus subject areas

  • Ocean Engineering

Cite this

Watras, C. J., Morrison, K. A., Rubsam, J. L., Hanson, P. C., Watras, A. J., Laliberte, G. D., & Milewski, P. (2017). A temperature compensation method for chlorophyll and phycocyanin fluorescence sensors in freshwater. DOI: 10.1002/lom3.10188

A temperature compensation method for chlorophyll and phycocyanin fluorescence sensors in freshwater. / Watras, C. J.; Morrison, K. A.; Rubsam, J. L.; Hanson, P. C.; Watras, A. J.; Laliberte, G. D.; Milewski, P.

In: Limnology and Oceanography: Methods, Vol. 15, No. 7, 07.2017, p. 642-652.

Research output: Contribution to journalArticle

Watras, CJ, Morrison, KA, Rubsam, JL, Hanson, PC, Watras, AJ, Laliberte, GD & Milewski, P 2017, 'A temperature compensation method for chlorophyll and phycocyanin fluorescence sensors in freshwater' Limnology and Oceanography: Methods, vol. 15, no. 7, pp. 642-652. DOI: 10.1002/lom3.10188
Watras CJ, Morrison KA, Rubsam JL, Hanson PC, Watras AJ, Laliberte GD et al. A temperature compensation method for chlorophyll and phycocyanin fluorescence sensors in freshwater. Limnology and Oceanography: Methods. 2017 Jul;15(7):642-652. Available from, DOI: 10.1002/lom3.10188
Watras, C. J. ; Morrison, K. A. ; Rubsam, J. L. ; Hanson, P. C. ; Watras, A. J. ; Laliberte, G. D. ; Milewski, P./ A temperature compensation method for chlorophyll and phycocyanin fluorescence sensors in freshwater. In: Limnology and Oceanography: Methods. 2017 ; Vol. 15, No. 7. pp. 642-652
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