A TCF-based fluorescent probe to determine nitroreductase (NTR) activity for a broad-spectrum of bacterial species

Kai Cheng Yan; Jordan E. Gardiner; Adam C. Sedgwick; Naing Thet; Rachel A. Heylen; Tony D. James; A. Toby A. Jenkins; Xiao Peng He

doi:10.1039/d3cc00462g

A TCF-based fluorescent probe to determine nitroreductase (NTR) activity for a broad-spectrum of bacterial species

Kai Cheng Yan, Jordan E. Gardiner, Adam C. Sedgwick, Naing Thet, Rachel A. Heylen, Tony D. James, A. Toby A. Jenkins, Xiao Peng He

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

3 Citations (SciVal)

Abstract

A nitroreductase (NTR) responsive fluorescent probe with long wavelength fluorescence emission was used to determine the NTR activity of a selection of bacterial species under a range of different bacterial growth conditions ensuring applicability under multiple complex clinical environments, where sensitivity, reaction time, and the detection accuracy were suitable for planktonic cultures and biofilms.

Original language	English
Pages (from-to)	8278-8281
Number of pages	4
Journal	Chemical Communications
Volume	59
Issue number	53
Early online date	15 Jun 2023
DOIs	https://doi.org/10.1039/d3cc00462g
Publication status	Published - 7 Jul 2023

Bibliographical note

X. P. H. wishes to thank the Natural National Science Foundation of China (No. 92253306), the Open Funding Project of the State Key Laboratory of Bioreactor Engineering of East China University of Science and Technology, the Fundamental Research Funds for the Central Universities (222201717003) and the Programme of Introducing Talents of Discipline to Universities (B16017) for financial support. A. T. A. J. would like to acknowledge funding from the EPSRC, grant reference: EP/V00462X/1. T. D. J. wishes to thank the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University for support (2020ZD01). R. A. H. thanks the Annette Trust and EPSRC for funding. A. C. S. would like to thank the Glasstone Research fellowship (University of Oxford) and the major research grant from Jesus College Oxford for financial support.

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Catalysis
Ceramics and Composites
General Chemistry
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

Access to Document

10.1039/d3cc00462gLicence: CC BY

Cite this

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title = "A TCF-based fluorescent probe to determine nitroreductase (NTR) activity for a broad-spectrum of bacterial species",

abstract = "A nitroreductase (NTR) responsive fluorescent probe with long wavelength fluorescence emission was used to determine the NTR activity of a selection of bacterial species under a range of different bacterial growth conditions ensuring applicability under multiple complex clinical environments, where sensitivity, reaction time, and the detection accuracy were suitable for planktonic cultures and biofilms.",

author = "Yan, {Kai Cheng} and Gardiner, {Jordan E.} and Sedgwick, {Adam C.} and Naing Thet and Heylen, {Rachel A.} and James, {Tony D.} and Jenkins, {A. Toby A.} and He, {Xiao Peng}",

note = "X. P. H. wishes to thank the Natural National Science Foundation of China (No. 92253306), the Open Funding Project of the State Key Laboratory of Bioreactor Engineering of East China University of Science and Technology, the Fundamental Research Funds for the Central Universities (222201717003) and the Programme of Introducing Talents of Discipline to Universities (B16017) for financial support. A. T. A. J. would like to acknowledge funding from the EPSRC, grant reference: EP/V00462X/1. T. D. J. wishes to thank the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University for support (2020ZD01). R. A. H. thanks the Annette Trust and EPSRC for funding. A. C. S. would like to thank the Glasstone Research fellowship (University of Oxford) and the major research grant from Jesus College Oxford for financial support.",

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AU - Gardiner, Jordan E.

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AU - Thet, Naing

AU - Heylen, Rachel A.

AU - James, Tony D.

AU - Jenkins, A. Toby A.

AU - He, Xiao Peng

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AB - A nitroreductase (NTR) responsive fluorescent probe with long wavelength fluorescence emission was used to determine the NTR activity of a selection of bacterial species under a range of different bacterial growth conditions ensuring applicability under multiple complex clinical environments, where sensitivity, reaction time, and the detection accuracy were suitable for planktonic cultures and biofilms.

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A TCF-based fluorescent probe to determine nitroreductase (NTR) activity for a broad-spectrum of bacterial species

Abstract

Bibliographical note

ASJC Scopus subject areas

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Plasma-activated antimicrobial hydrogel therapy (PAHT) for combating infections in diabetic foot ulcers - Bath-Lancaster-Glasgow project

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A TCF-based fluorescent probe to determine nitroreductase (NTR) activity for a broad-spectrum of bacterial species

Abstract

Bibliographical note

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Projects

Plasma-activated antimicrobial hydrogel therapy (PAHT) for combating infections in diabetic foot ulcers - Bath-Lancaster-Glasgow project

Cite this