A strategy to discover new organizers identifies a putative heart organizer

Claire Anderson, Mohsin A F Khan, Frances Wong, Tatiana Solovieva, Nidia M M Oliveira, Richard A. Baldock, Cheryll Tickle, Dave W. Burt, Claudio D. Stern

Research output: Contribution to journalArticle

4 Citations (Scopus)
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Abstract

Organizers are regions of the embryo that can both induce new fates and impart pattern on other regions. So far, surprisingly few organizers have been discovered, considering the number of patterned tissue types generated during development. This may be because their discovery has relied on transplantation and ablation experiments. Here we describe a new approach, using chick embryos, to discover organizers based on a common gene expression signature, and use it to uncover the anterior intestinal portal (AIP) endoderm as a putative heart organizer. We show that the AIP can induce cardiac identity from non-cardiac mesoderm and that it can pattern this by specifying ventricular and suppressing atrial regional identity. We also uncover some of the signals responsible. The method holds promise as a tool to discover other novel organizers acting during development.

Original languageEnglish
Article number12656
JournalNature Communications
Volume7
DOIs
Publication statusPublished - 25 Aug 2016

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Embryonic Organizers
Endoderm
embryos
Mesoderm
Chick Embryo
Transcriptome
Transplantation
transplantation
gene expression
Ablation
Gene expression
ablation
signatures
Tissue
Experiments

Cite this

Anderson, C., Khan, M. A. F., Wong, F., Solovieva, T., Oliveira, N. M. M., Baldock, R. A., ... Stern, C. D. (2016). A strategy to discover new organizers identifies a putative heart organizer. Nature Communications, 7, [12656]. https://doi.org/10.1038/ncomms12656

A strategy to discover new organizers identifies a putative heart organizer. / Anderson, Claire; Khan, Mohsin A F; Wong, Frances; Solovieva, Tatiana; Oliveira, Nidia M M; Baldock, Richard A.; Tickle, Cheryll; Burt, Dave W.; Stern, Claudio D.

In: Nature Communications, Vol. 7, 12656, 25.08.2016.

Research output: Contribution to journalArticle

Anderson, C, Khan, MAF, Wong, F, Solovieva, T, Oliveira, NMM, Baldock, RA, Tickle, C, Burt, DW & Stern, CD 2016, 'A strategy to discover new organizers identifies a putative heart organizer', Nature Communications, vol. 7, 12656. https://doi.org/10.1038/ncomms12656
Anderson C, Khan MAF, Wong F, Solovieva T, Oliveira NMM, Baldock RA et al. A strategy to discover new organizers identifies a putative heart organizer. Nature Communications. 2016 Aug 25;7. 12656. https://doi.org/10.1038/ncomms12656
Anderson, Claire ; Khan, Mohsin A F ; Wong, Frances ; Solovieva, Tatiana ; Oliveira, Nidia M M ; Baldock, Richard A. ; Tickle, Cheryll ; Burt, Dave W. ; Stern, Claudio D. / A strategy to discover new organizers identifies a putative heart organizer. In: Nature Communications. 2016 ; Vol. 7.
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