A strategy to determine operating parameters in tissue engineering hollow fiber bioreactors

R J Shipley, Adam J Davidson, K Chan, Julian B Chaudhuri, S L Waters, Marianne J Ellis

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The development of tissue engineering hollow fiber bioreactors (HFB) requires the optimal design of the geometry and operation parameters of the system. This article provides a strategy for specifying operating conditions for the system based on mathematical models of oxygen delivery to the cell population. Analytical and numerical solutions of these models are developed based on Michaelis-Menten kinetics. Depending on the minimum oxygen concentration required to culture a functional cell population, together with the oxygen uptake kinetics, the strategy dictates the model needed to describe mass transport so that the operating conditions can be defined. If c(min) >> K-m we capture oxygen uptake using zero-order kinetics and proceed analytically. This enables operating equations to be developed that allow the user to choose the medium flow rate, lumen length, and ECS depth to provide a prescribed value of c(min). When c(min) >> K-m, we use numerical techniques to solve full Michaelis-Menten kinetics and present operating data for the bioreactor. The strategy presented utilizes both analytical and numerical approaches and can be applied to any cell type with known oxygen transport properties and uptake kinetics.
Original languageEnglish
Pages (from-to)1450-1461
Number of pages12
JournalBiotechnology and Bioengineering
Volume108
Issue number6
Early online date2 Mar 2011
DOIs
Publication statusPublished - 1 Jun 2011

Keywords

  • mathematical modeling
  • tissue engineering
  • oxygen
  • mass transport
  • bioreactor

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