A specialized metabolic network selectively modulates Arabidopsis root microbiota

Ancheng C. Huang, Ting Jiang, Yong Xin Liu, Yue Chen Bai, James Reed, Baoyuan Qu, Alain Goossens, Hans Wilhelm Nützmann, Yang Bai, Anne Osbourn

Research output: Contribution to journalArticle

11 Citations (Scopus)
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Abstract

Plant specialized metabolites have ecological functions, yet the presence of numerous uncharacterized biosynthetic genes in plant genomes suggests that many molecules remain unknown.We discovered a triterpene biosynthetic network in the roots of the small mustard plant Arabidopsis thaliana. Collectively, we have elucidated and reconstituted three divergent pathways for the biosynthesis of root triterpenes, namely thalianin (seven steps), thalianyl medium-chain fatty acid esters (three steps), and arabidin (five steps). A. thaliana mutants disrupted in the biosynthesis of these compounds have altered root microbiota. In vitro bioassays with purified compounds reveal selective growth modulation activities of pathway metabolites toward root microbiota members and their biochemical transformation and utilization by bacteria, supporting a role for this biosynthetic network in shaping an Arabidopsis-specific root microbial community.

Original languageEnglish
Article numbereaau6389
JournalScience
Volume364
Issue number6440
DOIs
Publication statusPublished - 10 May 2019

ASJC Scopus subject areas

  • General

Cite this

Huang, A. C., Jiang, T., Liu, Y. X., Bai, Y. C., Reed, J., Qu, B., ... Osbourn, A. (2019). A specialized metabolic network selectively modulates Arabidopsis root microbiota. Science, 364(6440), [eaau6389]. https://doi.org/10.1126/science.aau6389

A specialized metabolic network selectively modulates Arabidopsis root microbiota. / Huang, Ancheng C.; Jiang, Ting; Liu, Yong Xin; Bai, Yue Chen; Reed, James; Qu, Baoyuan; Goossens, Alain; Nützmann, Hans Wilhelm; Bai, Yang; Osbourn, Anne.

In: Science, Vol. 364, No. 6440, eaau6389, 10.05.2019.

Research output: Contribution to journalArticle

Huang, AC, Jiang, T, Liu, YX, Bai, YC, Reed, J, Qu, B, Goossens, A, Nützmann, HW, Bai, Y & Osbourn, A 2019, 'A specialized metabolic network selectively modulates Arabidopsis root microbiota', Science, vol. 364, no. 6440, eaau6389. https://doi.org/10.1126/science.aau6389
Huang AC, Jiang T, Liu YX, Bai YC, Reed J, Qu B et al. A specialized metabolic network selectively modulates Arabidopsis root microbiota. Science. 2019 May 10;364(6440). eaau6389. https://doi.org/10.1126/science.aau6389
Huang, Ancheng C. ; Jiang, Ting ; Liu, Yong Xin ; Bai, Yue Chen ; Reed, James ; Qu, Baoyuan ; Goossens, Alain ; Nützmann, Hans Wilhelm ; Bai, Yang ; Osbourn, Anne. / A specialized metabolic network selectively modulates Arabidopsis root microbiota. In: Science. 2019 ; Vol. 364, No. 6440.
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AU - Goossens, Alain

AU - Nützmann, Hans Wilhelm

AU - Bai, Yang

AU - Osbourn, Anne

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