A single P450 allele associated with insecticide resistance in Drosophila

P J Daborn; J L Yen; M R Bogwitz; G Le Goff; E Feil; S Jeffers; N Tijet; T Perry; D Heckel; P Batterham; R Feyereisen; T G Wilson; R H ffrench-Constant

A single P450 allele associated with insecticide resistance in Drosophila

P J Daborn, J L Yen, M R Bogwitz, G Le Goff, E Feil, S Jeffers, N Tijet, T Perry, D Heckel, P Batterham, R Feyereisen, T G Wilson, R H ffrench-Constant

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

694 Citations (SciVal)

Abstract

Insecticide resistance is one of the most widespread genetic changes caused by human activity, but we still understand little about the origins and spread of resistant alleles in global populations of insects. Here, via microarray analysis of all P450s in Drosophila melanogaster, we show that DDT-R, a gene conferring resistance to DDT, is associated with overtranscription of a single cytochrome P450 gene, Cyp6g1. Transgenic analysis of Cyp6g1 shows that overtranscription of this gene alone is both necessary and sufficient for resistance. Resistance and up-regulation in Drosophila populations are associated with a single Cyp6g1 allele that has spread globally. This allele is characterized by the insertion of an Accord transposable element into the 5' end of the Cyp6g1 gene.

Original language	English
Pages (from-to)	2253-2256
Number of pages	4
Journal	Science
Volume	297
Issue number	5590
Publication status	Published - 2002

Bibliographical note

ID number: ISI:000178222000045

Cite this

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title = "A single P450 allele associated with insecticide resistance in Drosophila",

abstract = "Insecticide resistance is one of the most widespread genetic changes caused by human activity, but we still understand little about the origins and spread of resistant alleles in global populations of insects. Here, via microarray analysis of all P450s in Drosophila melanogaster, we show that DDT-R, a gene conferring resistance to DDT, is associated with overtranscription of a single cytochrome P450 gene, Cyp6g1. Transgenic analysis of Cyp6g1 shows that overtranscription of this gene alone is both necessary and sufficient for resistance. Resistance and up-regulation in Drosophila populations are associated with a single Cyp6g1 allele that has spread globally. This allele is characterized by the insertion of an Accord transposable element into the 5' end of the Cyp6g1 gene.",

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TY - JOUR

T1 - A single P450 allele associated with insecticide resistance in Drosophila

AU - Daborn, P J

AU - Yen, J L

AU - Bogwitz, M R

AU - Le Goff, G

AU - Feil, E

AU - Jeffers, S

AU - Tijet, N

AU - Perry, T

AU - Heckel, D

AU - Batterham, P

AU - Feyereisen, R

AU - Wilson, T G

AU - ffrench-Constant, R H

N1 - ID number: ISI:000178222000045

PY - 2002

Y1 - 2002

N2 - Insecticide resistance is one of the most widespread genetic changes caused by human activity, but we still understand little about the origins and spread of resistant alleles in global populations of insects. Here, via microarray analysis of all P450s in Drosophila melanogaster, we show that DDT-R, a gene conferring resistance to DDT, is associated with overtranscription of a single cytochrome P450 gene, Cyp6g1. Transgenic analysis of Cyp6g1 shows that overtranscription of this gene alone is both necessary and sufficient for resistance. Resistance and up-regulation in Drosophila populations are associated with a single Cyp6g1 allele that has spread globally. This allele is characterized by the insertion of an Accord transposable element into the 5' end of the Cyp6g1 gene.

AB - Insecticide resistance is one of the most widespread genetic changes caused by human activity, but we still understand little about the origins and spread of resistant alleles in global populations of insects. Here, via microarray analysis of all P450s in Drosophila melanogaster, we show that DDT-R, a gene conferring resistance to DDT, is associated with overtranscription of a single cytochrome P450 gene, Cyp6g1. Transgenic analysis of Cyp6g1 shows that overtranscription of this gene alone is both necessary and sufficient for resistance. Resistance and up-regulation in Drosophila populations are associated with a single Cyp6g1 allele that has spread globally. This allele is characterized by the insertion of an Accord transposable element into the 5' end of the Cyp6g1 gene.

M3 - Article

SN - 0036-8075

VL - 297

SP - 2253

EP - 2256

JO - Science

JF - Science

IS - 5590

ER -

A single P450 allele associated with insecticide resistance in Drosophila

Abstract

Bibliographical note

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