A Single Multilocus Sequence Typing (MLST) Scheme for Seven Pathogenic Leptospira Species

S. Boonsilp, J. Thaipadungpanit, P. Amornchai, V. Wuthiekanun, M.S. Bailey, M.T.G. Holden, C. Zhang, X. Jiang, N. Koizumi, K. Taylor, R. Galloway, A.R. Hoffmaster, S. Craig, L.D. Smythe, R.A. Hartskeerl, N.P. Day, N. Chantratita, E.J. Feil, D.M. Aanensen, B.G. Spratt & 1 others S.J. Peacock

Research output: Contribution to journalArticle

78 Citations (Scopus)
91 Downloads (Pure)

Abstract

Background: The available Leptospira multilocus sequence typing (MLST) scheme supported by a MLST website is limited to L. interrogans and L. kirschneri. Our aim was to broaden the utility of this scheme to incorporate a total of seven pathogenic species.

Methodology and Findings: We modified the existing scheme by replacing one of the seven MLST loci (fadD was changed to caiB), as the former gene did not appear to be present in some pathogenic species. Comparison of the original and modified schemes using data for L. interrogans and L. kirschneri demonstrated that the discriminatory power of the two schemes was not significantly different. The modified scheme was used to further characterize 325 isolates (L. alexanderi [n = 5], L. borgpetersenii [n = 34], L. interrogans [n = 222], L. kirschneri [n = 29], L. noguchii [n = 9], L. santarosai [n = 10], and L. weilii [n = 16]). Phylogenetic analysis using concatenated sequences of the 7 loci demonstrated that each species corresponded to a discrete clade, and that no strains were misclassified at the species level. Comparison between genotype and serovar was possible for 254 isolates. Of the 31 sequence types (STs) represented by at least two isolates, 18 STs included isolates assigned to two or three different serovars. Conversely, 14 serovars were identified that contained between 2 to 10 different STs. New observations were made on the global phylogeography of Leptospira spp., and the utility of MLST in making associations between human disease and specific maintenance hosts was demonstrated.

Conclusion: The new MLST scheme, supported by an updated MLST website, allows the characterization and species assignment of isolates of the seven major pathogenic species associated with leptospirosis.
Original languageEnglish
Article number1954
JournalPLoS Neglected Tropical Diseases
Volume7
Issue number1
DOIs
Publication statusPublished - Jan 2013

Fingerprint

Multilocus Sequence Typing
Leptospira
Phylogeography
Leptospirosis
Genotype
Maintenance
Genes
Serogroup

Cite this

Boonsilp, S., Thaipadungpanit, J., Amornchai, P., Wuthiekanun, V., Bailey, M. S., Holden, M. T. G., ... Peacock, S. J. (2013). A Single Multilocus Sequence Typing (MLST) Scheme for Seven Pathogenic Leptospira Species. PLoS Neglected Tropical Diseases, 7(1), [1954]. https://doi.org/10.1371/journal.pntd.0001954

A Single Multilocus Sequence Typing (MLST) Scheme for Seven Pathogenic Leptospira Species. / Boonsilp, S.; Thaipadungpanit, J.; Amornchai, P.; Wuthiekanun, V.; Bailey, M.S.; Holden, M.T.G.; Zhang, C.; Jiang, X.; Koizumi, N.; Taylor, K.; Galloway, R.; Hoffmaster, A.R.; Craig, S.; Smythe, L.D.; Hartskeerl, R.A.; Day, N.P.; Chantratita, N.; Feil, E.J.; Aanensen, D.M.; Spratt, B.G.; Peacock, S.J.

In: PLoS Neglected Tropical Diseases, Vol. 7, No. 1, 1954, 01.2013.

Research output: Contribution to journalArticle

Boonsilp, S, Thaipadungpanit, J, Amornchai, P, Wuthiekanun, V, Bailey, MS, Holden, MTG, Zhang, C, Jiang, X, Koizumi, N, Taylor, K, Galloway, R, Hoffmaster, AR, Craig, S, Smythe, LD, Hartskeerl, RA, Day, NP, Chantratita, N, Feil, EJ, Aanensen, DM, Spratt, BG & Peacock, SJ 2013, 'A Single Multilocus Sequence Typing (MLST) Scheme for Seven Pathogenic Leptospira Species', PLoS Neglected Tropical Diseases, vol. 7, no. 1, 1954. https://doi.org/10.1371/journal.pntd.0001954
Boonsilp S, Thaipadungpanit J, Amornchai P, Wuthiekanun V, Bailey MS, Holden MTG et al. A Single Multilocus Sequence Typing (MLST) Scheme for Seven Pathogenic Leptospira Species. PLoS Neglected Tropical Diseases. 2013 Jan;7(1). 1954. https://doi.org/10.1371/journal.pntd.0001954
Boonsilp, S. ; Thaipadungpanit, J. ; Amornchai, P. ; Wuthiekanun, V. ; Bailey, M.S. ; Holden, M.T.G. ; Zhang, C. ; Jiang, X. ; Koizumi, N. ; Taylor, K. ; Galloway, R. ; Hoffmaster, A.R. ; Craig, S. ; Smythe, L.D. ; Hartskeerl, R.A. ; Day, N.P. ; Chantratita, N. ; Feil, E.J. ; Aanensen, D.M. ; Spratt, B.G. ; Peacock, S.J. / A Single Multilocus Sequence Typing (MLST) Scheme for Seven Pathogenic Leptospira Species. In: PLoS Neglected Tropical Diseases. 2013 ; Vol. 7, No. 1.
@article{feb03d647c51420485d7a0a3f3be2271,
title = "A Single Multilocus Sequence Typing (MLST) Scheme for Seven Pathogenic Leptospira Species",
abstract = "Background: The available Leptospira multilocus sequence typing (MLST) scheme supported by a MLST website is limited to L. interrogans and L. kirschneri. Our aim was to broaden the utility of this scheme to incorporate a total of seven pathogenic species. Methodology and Findings: We modified the existing scheme by replacing one of the seven MLST loci (fadD was changed to caiB), as the former gene did not appear to be present in some pathogenic species. Comparison of the original and modified schemes using data for L. interrogans and L. kirschneri demonstrated that the discriminatory power of the two schemes was not significantly different. The modified scheme was used to further characterize 325 isolates (L. alexanderi [n = 5], L. borgpetersenii [n = 34], L. interrogans [n = 222], L. kirschneri [n = 29], L. noguchii [n = 9], L. santarosai [n = 10], and L. weilii [n = 16]). Phylogenetic analysis using concatenated sequences of the 7 loci demonstrated that each species corresponded to a discrete clade, and that no strains were misclassified at the species level. Comparison between genotype and serovar was possible for 254 isolates. Of the 31 sequence types (STs) represented by at least two isolates, 18 STs included isolates assigned to two or three different serovars. Conversely, 14 serovars were identified that contained between 2 to 10 different STs. New observations were made on the global phylogeography of Leptospira spp., and the utility of MLST in making associations between human disease and specific maintenance hosts was demonstrated. Conclusion: The new MLST scheme, supported by an updated MLST website, allows the characterization and species assignment of isolates of the seven major pathogenic species associated with leptospirosis.",
author = "S. Boonsilp and J. Thaipadungpanit and P. Amornchai and V. Wuthiekanun and M.S. Bailey and M.T.G. Holden and C. Zhang and X. Jiang and N. Koizumi and K. Taylor and R. Galloway and A.R. Hoffmaster and S. Craig and L.D. Smythe and R.A. Hartskeerl and N.P. Day and N. Chantratita and E.J. Feil and D.M. Aanensen and B.G. Spratt and S.J. Peacock",
year = "2013",
month = "1",
doi = "10.1371/journal.pntd.0001954",
language = "English",
volume = "7",
journal = "PLoS Neglected Tropical Diseases",
issn = "1935-2727",
publisher = "Public Library of Science (PLOS)",
number = "1",

}

TY - JOUR

T1 - A Single Multilocus Sequence Typing (MLST) Scheme for Seven Pathogenic Leptospira Species

AU - Boonsilp, S.

AU - Thaipadungpanit, J.

AU - Amornchai, P.

AU - Wuthiekanun, V.

AU - Bailey, M.S.

AU - Holden, M.T.G.

AU - Zhang, C.

AU - Jiang, X.

AU - Koizumi, N.

AU - Taylor, K.

AU - Galloway, R.

AU - Hoffmaster, A.R.

AU - Craig, S.

AU - Smythe, L.D.

AU - Hartskeerl, R.A.

AU - Day, N.P.

AU - Chantratita, N.

AU - Feil, E.J.

AU - Aanensen, D.M.

AU - Spratt, B.G.

AU - Peacock, S.J.

PY - 2013/1

Y1 - 2013/1

N2 - Background: The available Leptospira multilocus sequence typing (MLST) scheme supported by a MLST website is limited to L. interrogans and L. kirschneri. Our aim was to broaden the utility of this scheme to incorporate a total of seven pathogenic species. Methodology and Findings: We modified the existing scheme by replacing one of the seven MLST loci (fadD was changed to caiB), as the former gene did not appear to be present in some pathogenic species. Comparison of the original and modified schemes using data for L. interrogans and L. kirschneri demonstrated that the discriminatory power of the two schemes was not significantly different. The modified scheme was used to further characterize 325 isolates (L. alexanderi [n = 5], L. borgpetersenii [n = 34], L. interrogans [n = 222], L. kirschneri [n = 29], L. noguchii [n = 9], L. santarosai [n = 10], and L. weilii [n = 16]). Phylogenetic analysis using concatenated sequences of the 7 loci demonstrated that each species corresponded to a discrete clade, and that no strains were misclassified at the species level. Comparison between genotype and serovar was possible for 254 isolates. Of the 31 sequence types (STs) represented by at least two isolates, 18 STs included isolates assigned to two or three different serovars. Conversely, 14 serovars were identified that contained between 2 to 10 different STs. New observations were made on the global phylogeography of Leptospira spp., and the utility of MLST in making associations between human disease and specific maintenance hosts was demonstrated. Conclusion: The new MLST scheme, supported by an updated MLST website, allows the characterization and species assignment of isolates of the seven major pathogenic species associated with leptospirosis.

AB - Background: The available Leptospira multilocus sequence typing (MLST) scheme supported by a MLST website is limited to L. interrogans and L. kirschneri. Our aim was to broaden the utility of this scheme to incorporate a total of seven pathogenic species. Methodology and Findings: We modified the existing scheme by replacing one of the seven MLST loci (fadD was changed to caiB), as the former gene did not appear to be present in some pathogenic species. Comparison of the original and modified schemes using data for L. interrogans and L. kirschneri demonstrated that the discriminatory power of the two schemes was not significantly different. The modified scheme was used to further characterize 325 isolates (L. alexanderi [n = 5], L. borgpetersenii [n = 34], L. interrogans [n = 222], L. kirschneri [n = 29], L. noguchii [n = 9], L. santarosai [n = 10], and L. weilii [n = 16]). Phylogenetic analysis using concatenated sequences of the 7 loci demonstrated that each species corresponded to a discrete clade, and that no strains were misclassified at the species level. Comparison between genotype and serovar was possible for 254 isolates. Of the 31 sequence types (STs) represented by at least two isolates, 18 STs included isolates assigned to two or three different serovars. Conversely, 14 serovars were identified that contained between 2 to 10 different STs. New observations were made on the global phylogeography of Leptospira spp., and the utility of MLST in making associations between human disease and specific maintenance hosts was demonstrated. Conclusion: The new MLST scheme, supported by an updated MLST website, allows the characterization and species assignment of isolates of the seven major pathogenic species associated with leptospirosis.

UR - http://www.scopus.com/inward/record.url?scp=84873493253&partnerID=8YFLogxK

UR - http://dx.doi.org/10.1371/journal.pntd.0001954

U2 - 10.1371/journal.pntd.0001954

DO - 10.1371/journal.pntd.0001954

M3 - Article

VL - 7

JO - PLoS Neglected Tropical Diseases

JF - PLoS Neglected Tropical Diseases

SN - 1935-2727

IS - 1

M1 - 1954

ER -