Abstract
A simplified procedure for lipid digestion, well suited for handling a large number of samples, was used to analyze a variety of common phospholipids. This procedure involves digestion of phospholipids in perchloric acid at 130°C with minimal sample manipulation. For all lipids tested, complete destruction, needed for quantitation of phosphate, was achieved after a few hours of digestion under these conditions. Rates of phospholipid destruction, monitored by the spectrophotometric quantitation of released phosphate, varied with lipid structure. Phosphatidic acid (PA), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG) and phospatidylinositol (PI) were found to release phosphate faster than phosphatidylserine (PS), phosphatidylethanolamine (PE) and phosphatidylcholine (PC). Although these differences may vary depending on the digestion conditions, they suggest that care should be exercised in lipid phosphate analyses to insure complete digestion.
| Original language | English |
|---|---|
| Pages (from-to) | 185-191 |
| Number of pages | 7 |
| Journal | Chemistry and Physics of Lipids |
| Volume | 39 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - 31 Jan 1986 |
Funding
We are grateful to Drs. John Silvius and Patricia Jost and Debra McMillen for useful suggestions. This work has been supported by PHS grant GM25698 from the National Institutes of Health. R.J.M. was the recipient of NIH postdoctoral fellowship GM08712.
| Funders | Funder number |
|---|---|
| National Institutes of Health | GM08712 |
| National Institutes of Health- National Institute of General Medical Sciences (USA) | R01GM025698 |
| Presbyterian Historical Society |
Keywords
- phosphate analysis
- phospholipid digestion
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Organic Chemistry
- Cell Biology