A simple umbelliferone based fluorescent probe for the detection of nitroreductase

Adam C. Sedgwick; Alex Hayden; Barry Hill; Steven D. Bull; Robert B.P. Elmes; Tony D. James

doi:10.1007/s11705-017-1697-0

A simple umbelliferone based fluorescent probe for the detection of nitroreductase

Adam C. Sedgwick, Alex Hayden, Barry Hill, Steven D. Bull, Robert B.P. Elmes, Tony D. James

Department of Chemistry

Maynooth University

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Abstract

A simple nitrobenzyl-umbelliferone (NCOU1) was synthesised containing a nitroreductase (NTR) trigger moiety. The presence of NTR, resulted in the fragmentation of the parent molecule and release of the highly emissive fluorophore umbelliferone via an NTR-catalyzed reduction of the nitro group. In the presence of the NTR enzyme, NCOU1 gave rise to a 5-fold increase in fluorescence intensity at 455 nm and was selective for NTR over other reductive enzymes. These results indicate that NCOU1 can be used as a simple assay for the detection of NTR. [Figure not available: see fulltext.]

Original language	English
Pages (from-to)	311-314
Number of pages	4
Journal	Frontiers of Chemical Science and Engineering
Volume	12
Issue number	2
Early online date	17 Jan 2018
DOIs	https://doi.org/10.1007/s11705-017-1697-0
Publication status	Published - 1 Jun 2018

ASJC Scopus subject areas

General Chemical Engineering

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title = "A simple umbelliferone based fluorescent probe for the detection of nitroreductase",

abstract = "A simple nitrobenzyl-umbelliferone (NCOU1) was synthesised containing a nitroreductase (NTR) trigger moiety. The presence of NTR, resulted in the fragmentation of the parent molecule and release of the highly emissive fluorophore umbelliferone via an NTR-catalyzed reduction of the nitro group. In the presence of the NTR enzyme, NCOU1 gave rise to a 5-fold increase in fluorescence intensity at 455 nm and was selective for NTR over other reductive enzymes. These results indicate that NCOU1 can be used as a simple assay for the detection of NTR. [Figure not available: see fulltext.]",

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T1 - A simple umbelliferone based fluorescent probe for the detection of nitroreductase

AU - Sedgwick, Adam C.

AU - Hayden, Alex

AU - Hill, Barry

AU - Bull, Steven D.

AU - Elmes, Robert B.P.

AU - James, Tony D.

PY - 2018/6/1

Y1 - 2018/6/1

N2 - A simple nitrobenzyl-umbelliferone (NCOU1) was synthesised containing a nitroreductase (NTR) trigger moiety. The presence of NTR, resulted in the fragmentation of the parent molecule and release of the highly emissive fluorophore umbelliferone via an NTR-catalyzed reduction of the nitro group. In the presence of the NTR enzyme, NCOU1 gave rise to a 5-fold increase in fluorescence intensity at 455 nm and was selective for NTR over other reductive enzymes. These results indicate that NCOU1 can be used as a simple assay for the detection of NTR. [Figure not available: see fulltext.]

AB - A simple nitrobenzyl-umbelliferone (NCOU1) was synthesised containing a nitroreductase (NTR) trigger moiety. The presence of NTR, resulted in the fragmentation of the parent molecule and release of the highly emissive fluorophore umbelliferone via an NTR-catalyzed reduction of the nitro group. In the presence of the NTR enzyme, NCOU1 gave rise to a 5-fold increase in fluorescence intensity at 455 nm and was selective for NTR over other reductive enzymes. These results indicate that NCOU1 can be used as a simple assay for the detection of NTR. [Figure not available: see fulltext.]

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JO - Frontiers of Chemical Science and Engineering

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A simple umbelliferone based fluorescent probe for the detection of nitroreductase

Abstract

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