Abstract
A simple nitrobenzyl-umbelliferone (NCOU1) was synthesised containing a nitroreductase (NTR) trigger moiety. The presence of NTR, resulted in the fragmentation of the parent molecule and release of the highly emissive fluorophore umbelliferone via an NTR-catalyzed reduction of the nitro group. In the presence of the NTR enzyme, NCOU1 gave rise to a 5-fold increase in fluorescence intensity at 455 nm and was selective for NTR over other reductive enzymes. These results indicate that NCOU1 can be used as a simple assay for the detection of NTR. [Figure not available: see fulltext.]
Original language | English |
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Pages (from-to) | 311-314 |
Number of pages | 4 |
Journal | Frontiers of Chemical Science and Engineering |
Volume | 12 |
Issue number | 2 |
Early online date | 17 Jan 2018 |
DOIs | |
Publication status | Published - 1 Jun 2018 |
ASJC Scopus subject areas
- General Chemical Engineering