A simple umbelliferone based fluorescent probe for the detection of nitroreductase

Adam C. Sedgwick, Alex Hayden, Barry Hill, Steven D. Bull, Robert B.P. Elmes, Tony D. James

Research output: Contribution to journalArticle

Abstract

A simple nitrobenzyl-umbelliferone (NCOU1) was synthesised containing a nitroreductase (NTR) trigger moiety. The presence of NTR, resulted in the fragmentation of the parent molecule and release of the highly emissive fluorophore umbelliferone via an NTR-catalyzed reduction of the nitro group. In the presence of the NTR enzyme, NCOU1 gave rise to a 5-fold increase in fluorescence intensity at 455 nm and was selective for NTR over other reductive enzymes. These results indicate that NCOU1 can be used as a simple assay for the detection of NTR. [Figure not available: see fulltext.]

LanguageEnglish
Pages311-314
Number of pages4
JournalFrontiers of Chemical Science and Engineering
Volume12
Issue number2
Early online date17 Jan 2018
DOIs
StatusPublished - 1 Jun 2018

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7-hydroxycoumarin
Nitroreductases
Fluorescent Dyes
Enzymes
Fluorophores
Assays
Fluorescence
Molecules

ASJC Scopus subject areas

  • Chemical Engineering(all)

Cite this

A simple umbelliferone based fluorescent probe for the detection of nitroreductase. / Sedgwick, Adam C.; Hayden, Alex; Hill, Barry; Bull, Steven D.; Elmes, Robert B.P.; James, Tony D.

In: Frontiers of Chemical Science and Engineering, Vol. 12, No. 2, 01.06.2018, p. 311-314.

Research output: Contribution to journalArticle

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