A simple device for multiplex ELISA made from melt-extruded plastic microcapillary film

Alexander D. Edwards, Nuno M. Reis, Nigel K. H. Slater, Malcolm R. Mackley

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract



We present a simple device for multiplex quantitative enzyme-linked immunosorbant assays (ELISA) made from a novel melt-extruded microcapillary film (MCF) containing a parallel array of 200μm capillaries along its length. To make ELISA devices different protein antigens or antibodies were immobilised inside individual microcapillaries within long reels of MCF extruded from fluorinated ethylene propylene (FEP). Short pieces of coated film were cut and interfaced with a pipette, allowing sequential uptake of samples and detection solutions into all capillaries from a reagent well. As well as being simple to produce, these FEP MCF devices have excellent light transmittance allowing direct optical interrogation of the capillaries for simple signal quantification. Proof of concept experiments demonstrate both quantitative and multiplex assays in FEP MCF devices using a standard direct ELISA procedure and read using a flatbed scanner. This new multiplex immunoassay platform should find applications ranging from lab detection to point-of-care and field diagnostics.
Original languageEnglish
Pages (from-to)4267-4273
JournalLab on a Chip
Volume11
Issue number24
DOIs
Publication statusPublished - 21 Dec 2011

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Plastic films
Plastics
Assays
Enzymes
Equipment and Supplies
Propylene
Ethylene
Point-of-Care Systems
Immobilized Antibodies
Immunoassay
Reels
Antigens
Antibodies
Light
Proteins
fluorinated ethylene propylene
Experiments

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A simple device for multiplex ELISA made from melt-extruded plastic microcapillary film. / Edwards, Alexander D.; Reis, Nuno M.; Slater, Nigel K. H.; Mackley, Malcolm R.

In: Lab on a Chip, Vol. 11, No. 24, 21.12.2011, p. 4267-4273.

Research output: Contribution to journalArticle

Edwards, Alexander D. ; Reis, Nuno M. ; Slater, Nigel K. H. ; Mackley, Malcolm R. / A simple device for multiplex ELISA made from melt-extruded plastic microcapillary film. In: Lab on a Chip. 2011 ; Vol. 11, No. 24. pp. 4267-4273.
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