A simple coumarin-based system for the effective detection of superoxide in bacteria

Kai-Cheng Yan; Qiao Cao; Naing Thet; Adam C. Sedgwick; Tony D. James; A. Toby A. Jenkins

doi:10.1016/j.rechem.2023.101244

A simple coumarin-based system for the effective detection of superoxide in bacteria

Kai-Cheng Yan, Qiao Cao, Naing Thet, Adam C. Sedgwick, Tony D. James, A. Toby A. Jenkins

University of Oxford

Research output: Contribution to journal › Article › peer-review

Abstract

Superoxide anion is a highly reactive ROS however the sensing of this species is relatively unexplored. The current methods to sense superoxide requires the construction of complex probes which are often hard to synthesize. With this research we report a simple one step high yielding (90%) method to prepare a probe for the detection of O2.- in bacteria using a coumarin core and trifluoromethanesulfonate locking group.

Original language	English
Article number	101244
Journal	Results in Chemistry
Volume	7
Early online date	2 Dec 2023
DOIs	https://doi.org/10.1016/j.rechem.2023.101244
Publication status	Published - 31 Jan 2024

Bibliographical note

Data availability:
All data supporting this study are provided as supplementary information accompanying this paper.

Funding

KCY and QC contributed equally to this work. QC thanks University of Bath for a Global Doctoral Scholarship and the UCB Biopharma for generous financial support. ACS would like to thank the Glasstone Research fellowship (University of Oxford) and the major research grant from Jesus College Oxford for financial support. ATAJ and NT would like to acknowledge funding from the EPSRC, grant reference: EP/V00462X/1. TDJ wishes to thank the Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University for support (2020ZD01).

Funders	Funder number
Jesus College Oxford
UCB Biopharma
Henan Normal University	2020ZD01
Engineering and Physical Sciences Research Council	EP/V00462X/1
University of Oxford
University of Bath

Keywords

Bacteria
Fluorescent probe
Superoxide

ASJC Scopus subject areas

General Chemistry

Access to Document

10.1016/j.rechem.2023.101244Licence: CC BY

https://linkinghub.elsevier.com/retrieve/pii/S2211715623004836

Cite this

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title = "A simple coumarin-based system for the effective detection of superoxide in bacteria",

abstract = "Superoxide anion is a highly reactive ROS however the sensing of this species is relatively unexplored. The current methods to sense superoxide requires the construction of complex probes which are often hard to synthesize. With this research we report a simple one step high yielding (90%) method to prepare a probe for the detection of O2.- in bacteria using a coumarin core and trifluoromethanesulfonate locking group.",

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AU - Cao, Qiao

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AU - James, Tony D.

AU - Jenkins, A. Toby A.

N1 - Data availability: All data supporting this study are provided as supplementary information accompanying this paper.

PY - 2024/1/31

Y1 - 2024/1/31

N2 - Superoxide anion is a highly reactive ROS however the sensing of this species is relatively unexplored. The current methods to sense superoxide requires the construction of complex probes which are often hard to synthesize. With this research we report a simple one step high yielding (90%) method to prepare a probe for the detection of O2.- in bacteria using a coumarin core and trifluoromethanesulfonate locking group.

AB - Superoxide anion is a highly reactive ROS however the sensing of this species is relatively unexplored. The current methods to sense superoxide requires the construction of complex probes which are often hard to synthesize. With this research we report a simple one step high yielding (90%) method to prepare a probe for the detection of O2.- in bacteria using a coumarin core and trifluoromethanesulfonate locking group.

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A simple coumarin-based system for the effective detection of superoxide in bacteria

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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Plasma-activated antimicrobial hydrogel therapy (PAHT) for combating infections in diabetic foot ulcers - Bath-Lancaster-Glasgow project

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A simple coumarin-based system for the effective detection of superoxide in bacteria

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Projects

Plasma-activated antimicrobial hydrogel therapy (PAHT) for combating infections in diabetic foot ulcers - Bath-Lancaster-Glasgow project

Cite this