A simple and highly sensitive electrochemical platform for detection of microRNAs

Pawan Jolly, Lai Chun Caleb Wong, Anna Miodek, Mark Lindsay, Pedro Estrela

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)
101 Downloads (Pure)

Abstract

MicroRNAs (miRNAs) in blood can act as fingerprints to many diseases including cancer. However, detection of low levels of these miRNAs in blood demands for
very sensitive techniques. Despite many reports available for miRNA detection, the available methodologies have drawbacks such as complexity, use of expensive enzymes, etc. We here report a new and simple electrochemical method to detect miRNAs by exploiting the intrinsic charges of the oligonucleotides and a simple amplification method with positively charged gold nanoparticles (AuNPs). Electrochemical impedance spectroscopy (EIS) was used to monitor the changes in capacitance upon miRNA binding without the need for redox markers. miRNA detection was also obtained through measurements of open circuit potential (OCP) variations. We demonstrate a promising cost effective biosensor using both EIS and OCP with a detection limit of 1 fM, which can easily be expanded into miRNA/DNA microarray platforms.
Original languageEnglish
Title of host publicationProceedings of IEEE Sensors 2015
PublisherIEEE
Pages803-806
Number of pages4
ISBN (Print)9781479982035
DOIs
Publication statusPublished - 2015
Event2015 IEEE Sensors - Busan, Korea, Republic of
Duration: 1 Nov 20154 Nov 2015

Conference

Conference2015 IEEE Sensors
CountryKorea, Republic of
CityBusan
Period1/11/154/11/15

Fingerprint

MicroRNAs
Electrochemical impedance spectroscopy
Blood
Networks (circuits)
Microarrays
Biosensors
Oligonucleotides
Gold
Amplification
Capacitance
Nanoparticles
DNA
Enzymes
Costs

Cite this

Jolly, P., Wong, L. C. C., Miodek, A., Lindsay, M., & Estrela, P. (2015). A simple and highly sensitive electrochemical platform for detection of microRNAs. In Proceedings of IEEE Sensors 2015 (pp. 803-806). IEEE. https://doi.org/10.1109/ICSENS.2015.7370378

A simple and highly sensitive electrochemical platform for detection of microRNAs. / Jolly, Pawan; Wong, Lai Chun Caleb; Miodek, Anna; Lindsay, Mark; Estrela, Pedro.

Proceedings of IEEE Sensors 2015. IEEE, 2015. p. 803-806.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Jolly, P, Wong, LCC, Miodek, A, Lindsay, M & Estrela, P 2015, A simple and highly sensitive electrochemical platform for detection of microRNAs. in Proceedings of IEEE Sensors 2015. IEEE, pp. 803-806, 2015 IEEE Sensors, Busan, Korea, Republic of, 1/11/15. https://doi.org/10.1109/ICSENS.2015.7370378
Jolly, Pawan ; Wong, Lai Chun Caleb ; Miodek, Anna ; Lindsay, Mark ; Estrela, Pedro. / A simple and highly sensitive electrochemical platform for detection of microRNAs. Proceedings of IEEE Sensors 2015. IEEE, 2015. pp. 803-806
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