A simple and highly sensitive electrochemical platform for detection of microRNAs

Pawan Jolly; Lai Chun Caleb Wong; Anna Miodek; Mark Lindsay; Pedro Estrela

doi:10.1109/ICSENS.2015.7370378

A simple and highly sensitive electrochemical platform for detection of microRNAs

Pawan Jolly, Lai Chun Caleb Wong, Anna Miodek, Mark Lindsay, Pedro Estrela

Research output: Chapter or section in a book/report/conference proceeding › Chapter in a published conference proceeding

6 Citations (SciVal)

293 Downloads (Pure)

Abstract

MicroRNAs (miRNAs) in blood can act as fingerprints to many diseases including cancer. However, detection of low levels of these miRNAs in blood demands for
very sensitive techniques. Despite many reports available for miRNA detection, the available methodologies have drawbacks such as complexity, use of expensive enzymes, etc. We here report a new and simple electrochemical method to detect miRNAs by exploiting the intrinsic charges of the oligonucleotides and a simple amplification method with positively charged gold nanoparticles (AuNPs). Electrochemical impedance spectroscopy (EIS) was used to monitor the changes in capacitance upon miRNA binding without the need for redox markers. miRNA detection was also obtained through measurements of open circuit potential (OCP) variations. We demonstrate a promising cost effective biosensor using both EIS and OCP with a detection limit of 1 fM, which can easily be expanded into miRNA/DNA microarray platforms.

Original language	English
Title of host publication	Proceedings of IEEE Sensors 2015
Publisher	IEEE
Pages	803-806
Number of pages	4
ISBN (Print)	9781479982035
DOIs	https://doi.org/10.1109/ICSENS.2015.7370378
Publication status	Published - 2015
Event	2015 IEEE Sensors - Busan, Korea, Republic of Duration: 1 Nov 2015 → 4 Nov 2015

Conference

Conference	2015 IEEE Sensors
Country/Territory	Korea, Republic of
City	Busan
Period	1/11/15 → 4/11/15

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1109/ICSENS.2015.7370378

IEEE paper final
© 2015 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other users, including reprinting/ republishing this material for advertising or promotional purposes, creating new collective works for resale or redistribution to servers or lists, or reuse of any copyrighted components of this work in other works.
Submitted manuscript, 716 KB

Cite this

@inproceedings{e5aa8f8e70514ed58d1f6cc88c74e3f3,

title = "A simple and highly sensitive electrochemical platform for detection of microRNAs",

abstract = "MicroRNAs (miRNAs) in blood can act as fingerprints to many diseases including cancer. However, detection of low levels of these miRNAs in blood demands forvery sensitive techniques. Despite many reports available for miRNA detection, the available methodologies have drawbacks such as complexity, use of expensive enzymes, etc. We here report a new and simple electrochemical method to detect miRNAs by exploiting the intrinsic charges of the oligonucleotides and a simple amplification method with positively charged gold nanoparticles (AuNPs). Electrochemical impedance spectroscopy (EIS) was used to monitor the changes in capacitance upon miRNA binding without the need for redox markers. miRNA detection was also obtained through measurements of open circuit potential (OCP) variations. We demonstrate a promising cost effective biosensor using both EIS and OCP with a detection limit of 1 fM, which can easily be expanded into miRNA/DNA microarray platforms.",

author = "Pawan Jolly and Wong, {Lai Chun Caleb} and Anna Miodek and Mark Lindsay and Pedro Estrela",

year = "2015",

doi = "10.1109/ICSENS.2015.7370378",

language = "English",

isbn = "9781479982035",

pages = "803--806",

booktitle = "Proceedings of IEEE Sensors 2015",

publisher = "IEEE",

address = "USA United States",

note = "2015 IEEE Sensors ; Conference date: 01-11-2015 Through 04-11-2015",

}

TY - GEN

T1 - A simple and highly sensitive electrochemical platform for detection of microRNAs

AU - Jolly, Pawan

AU - Wong, Lai Chun Caleb

AU - Miodek, Anna

AU - Lindsay, Mark

AU - Estrela, Pedro

PY - 2015

Y1 - 2015

N2 - MicroRNAs (miRNAs) in blood can act as fingerprints to many diseases including cancer. However, detection of low levels of these miRNAs in blood demands forvery sensitive techniques. Despite many reports available for miRNA detection, the available methodologies have drawbacks such as complexity, use of expensive enzymes, etc. We here report a new and simple electrochemical method to detect miRNAs by exploiting the intrinsic charges of the oligonucleotides and a simple amplification method with positively charged gold nanoparticles (AuNPs). Electrochemical impedance spectroscopy (EIS) was used to monitor the changes in capacitance upon miRNA binding without the need for redox markers. miRNA detection was also obtained through measurements of open circuit potential (OCP) variations. We demonstrate a promising cost effective biosensor using both EIS and OCP with a detection limit of 1 fM, which can easily be expanded into miRNA/DNA microarray platforms.

AB - MicroRNAs (miRNAs) in blood can act as fingerprints to many diseases including cancer. However, detection of low levels of these miRNAs in blood demands forvery sensitive techniques. Despite many reports available for miRNA detection, the available methodologies have drawbacks such as complexity, use of expensive enzymes, etc. We here report a new and simple electrochemical method to detect miRNAs by exploiting the intrinsic charges of the oligonucleotides and a simple amplification method with positively charged gold nanoparticles (AuNPs). Electrochemical impedance spectroscopy (EIS) was used to monitor the changes in capacitance upon miRNA binding without the need for redox markers. miRNA detection was also obtained through measurements of open circuit potential (OCP) variations. We demonstrate a promising cost effective biosensor using both EIS and OCP with a detection limit of 1 fM, which can easily be expanded into miRNA/DNA microarray platforms.

UR - http://dx.doi.org/10.1109/ICSENS.2015.7370378

U2 - 10.1109/ICSENS.2015.7370378

DO - 10.1109/ICSENS.2015.7370378

M3 - Chapter in a published conference proceeding

SN - 9781479982035

SP - 803

EP - 806

BT - Proceedings of IEEE Sensors 2015

PB - IEEE

T2 - 2015 IEEE Sensors

Y2 - 1 November 2015 through 4 November 2015

ER -

A simple and highly sensitive electrochemical platform for detection of microRNAs

Abstract

Conference

UN SDGs

Access to Document

Other files and links

Fingerprint

Cancer Diagnosis: Parallel Sensing of Prostate Cancer Biomarkers: MARIE CURIE - PROSENSE Training budget

Cite this

A simple and highly sensitive electrochemical platform for detection of microRNAs

Abstract

Conference

UN SDGs

Access to Document

Other files and links

Fingerprint

Projects

Cancer Diagnosis: Parallel Sensing of Prostate Cancer Biomarkers: MARIE CURIE - PROSENSE Training budget

Cite this