A set of ninety-seven overlapping yeast artificial chromosome clones spanning the human Y chromosome euchromatin

Michael H. Jones, Omar S. A. Khwaja, Hayely. Briggs, Bronwen Lambson, Phillip M. Davey, Jennifer Chalmers, Chun-Yan Zhou, Elizabeth M. Walker, Yan Zhang, Chris Todd, Malcolm A. Ferguson-Smith, Nabeel A. Affara

Research output: Contribution to journalArticle

Abstract

Contiguous arrays of yeast artificial chromosomes (YACs) extending from proximal heterochromatic Yq into the pseudoautosomal portion of the Y chromosome and separated by a small interval at the centromere have been constructed. A total of 97 YACs have been aligned along the Y chromosome by STS content analysis using 222 sequence tagged sites (STSs) that detect 263 loci. Forty-five of the STSs used are novel. Their inclusion provides a significant improvement over previously available maps on the density of STS coverage along the Y chromosome, reducing the average spacing to 120 kb assuming a length of 30 Mb for the euchromatin. The average size of 61 YACs determined by pulsed-field gel electrophoresis analysis was at least 0.9 Mb. Minor differences noted between the ordering of STSs on this map compared with those previously reported may be attributed to inherent polymorphism between the Y chromosomes used to construct the YAC libraries.
Original languageEnglish
Pages (from-to)266-275
Number of pages10
JournalGenomics
Volume24
Issue number2
DOIs
Publication statusPublished - 23 Apr 1994

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Chromosomes, Human, Y
Sequence Tagged Sites
Euchromatin
Yeast Artificial Chromosomes
Y Chromosome
Clone Cells
Centromere
Pulsed Field Gel Electrophoresis

Keywords

  • article
  • autosome
  • euchromatin
  • gene mapping
  • genetic polymorphism
  • molecular cloning
  • nonhuman
  • priority journal
  • sequence tagged site
  • Y chromosome
  • yeast artificial chromosome

Cite this

Jones, M. H., Khwaja, O. S. A., Briggs, H., Lambson, B., Davey, P. M., Chalmers, J., ... Affara, N. A. (1994). A set of ninety-seven overlapping yeast artificial chromosome clones spanning the human Y chromosome euchromatin. Genomics, 24(2), 266-275. https://doi.org/10.1006/geno.1994.1615

A set of ninety-seven overlapping yeast artificial chromosome clones spanning the human Y chromosome euchromatin. / Jones, Michael H.; Khwaja, Omar S. A.; Briggs, Hayely.; Lambson, Bronwen; Davey, Phillip M.; Chalmers, Jennifer; Zhou, Chun-Yan; Walker, Elizabeth M.; Zhang, Yan; Todd, Chris; Ferguson-Smith, Malcolm A.; Affara, Nabeel A.

In: Genomics, Vol. 24, No. 2, 23.04.1994, p. 266-275.

Research output: Contribution to journalArticle

Jones, MH, Khwaja, OSA, Briggs, H, Lambson, B, Davey, PM, Chalmers, J, Zhou, C-Y, Walker, EM, Zhang, Y, Todd, C, Ferguson-Smith, MA & Affara, NA 1994, 'A set of ninety-seven overlapping yeast artificial chromosome clones spanning the human Y chromosome euchromatin', Genomics, vol. 24, no. 2, pp. 266-275. https://doi.org/10.1006/geno.1994.1615
Jones, Michael H. ; Khwaja, Omar S. A. ; Briggs, Hayely. ; Lambson, Bronwen ; Davey, Phillip M. ; Chalmers, Jennifer ; Zhou, Chun-Yan ; Walker, Elizabeth M. ; Zhang, Yan ; Todd, Chris ; Ferguson-Smith, Malcolm A. ; Affara, Nabeel A. / A set of ninety-seven overlapping yeast artificial chromosome clones spanning the human Y chromosome euchromatin. In: Genomics. 1994 ; Vol. 24, No. 2. pp. 266-275.
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