A set of ninety-seven overlapping yeast artificial chromosome clones spanning the human Y chromosome euchromatin

Michael H. Jones; Omar S. A. Khwaja; Hayely. Briggs; Bronwen Lambson; Phillip M. Davey; Jennifer Chalmers; Chun-Yan Zhou; Elizabeth M. Walker; Yan Zhang; Chris Todd; Malcolm A. Ferguson-Smith; Nabeel A. Affara

doi:10.1006/geno.1994.1615

A set of ninety-seven overlapping yeast artificial chromosome clones spanning the human Y chromosome euchromatin

Michael H. Jones, Omar S. A. Khwaja, Hayely. Briggs, Bronwen Lambson, Phillip M. Davey, Jennifer Chalmers, Chun-Yan Zhou, Elizabeth M. Walker, Yan Zhang, Chris Todd, Malcolm A. Ferguson-Smith, Nabeel A. Affara

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Contiguous arrays of yeast artificial chromosomes (YACs) extending from proximal heterochromatic Yq into the pseudoautosomal portion of the Y chromosome and separated by a small interval at the centromere have been constructed. A total of 97 YACs have been aligned along the Y chromosome by STS content analysis using 222 sequence tagged sites (STSs) that detect 263 loci. Forty-five of the STSs used are novel. Their inclusion provides a significant improvement over previously available maps on the density of STS coverage along the Y chromosome, reducing the average spacing to 120 kb assuming a length of 30 Mb for the euchromatin. The average size of 61 YACs determined by pulsed-field gel electrophoresis analysis was at least 0.9 Mb. Minor differences noted between the ordering of STSs on this map compared with those previously reported may be attributed to inherent polymorphism between the Y chromosomes used to construct the YAC libraries.

Original language	English
Pages (from-to)	266-275
Number of pages	10
Journal	Genomics
Volume	24
Issue number	2
DOIs	https://doi.org/10.1006/geno.1994.1615
Publication status	Published - 23 Apr 1994

Keywords

article
autosome
euchromatin
gene mapping
genetic polymorphism
molecular cloning
nonhuman
priority journal
sequence tagged site
Y chromosome
yeast artificial chromosome

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10.1006/geno.1994.1615

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title = "A set of ninety-seven overlapping yeast artificial chromosome clones spanning the human Y chromosome euchromatin",

abstract = "Contiguous arrays of yeast artificial chromosomes (YACs) extending from proximal heterochromatic Yq into the pseudoautosomal portion of the Y chromosome and separated by a small interval at the centromere have been constructed. A total of 97 YACs have been aligned along the Y chromosome by STS content analysis using 222 sequence tagged sites (STSs) that detect 263 loci. Forty-five of the STSs used are novel. Their inclusion provides a significant improvement over previously available maps on the density of STS coverage along the Y chromosome, reducing the average spacing to 120 kb assuming a length of 30 Mb for the euchromatin. The average size of 61 YACs determined by pulsed-field gel electrophoresis analysis was at least 0.9 Mb. Minor differences noted between the ordering of STSs on this map compared with those previously reported may be attributed to inherent polymorphism between the Y chromosomes used to construct the YAC libraries.",

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author = "Jones, {Michael H.} and Khwaja, {Omar S. A.} and Hayely. Briggs and Bronwen Lambson and Davey, {Phillip M.} and Jennifer Chalmers and Chun-Yan Zhou and Walker, {Elizabeth M.} and Yan Zhang and Chris Todd and Ferguson-Smith, {Malcolm A.} and Affara, {Nabeel A.}",

year = "1994",

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doi = "10.1006/geno.1994.1615",

language = "English",

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AU - Briggs, Hayely.

AU - Lambson, Bronwen

AU - Davey, Phillip M.

AU - Chalmers, Jennifer

AU - Zhou, Chun-Yan

AU - Walker, Elizabeth M.

AU - Zhang, Yan

AU - Todd, Chris

AU - Ferguson-Smith, Malcolm A.

AU - Affara, Nabeel A.

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N2 - Contiguous arrays of yeast artificial chromosomes (YACs) extending from proximal heterochromatic Yq into the pseudoautosomal portion of the Y chromosome and separated by a small interval at the centromere have been constructed. A total of 97 YACs have been aligned along the Y chromosome by STS content analysis using 222 sequence tagged sites (STSs) that detect 263 loci. Forty-five of the STSs used are novel. Their inclusion provides a significant improvement over previously available maps on the density of STS coverage along the Y chromosome, reducing the average spacing to 120 kb assuming a length of 30 Mb for the euchromatin. The average size of 61 YACs determined by pulsed-field gel electrophoresis analysis was at least 0.9 Mb. Minor differences noted between the ordering of STSs on this map compared with those previously reported may be attributed to inherent polymorphism between the Y chromosomes used to construct the YAC libraries.

AB - Contiguous arrays of yeast artificial chromosomes (YACs) extending from proximal heterochromatic Yq into the pseudoautosomal portion of the Y chromosome and separated by a small interval at the centromere have been constructed. A total of 97 YACs have been aligned along the Y chromosome by STS content analysis using 222 sequence tagged sites (STSs) that detect 263 loci. Forty-five of the STSs used are novel. Their inclusion provides a significant improvement over previously available maps on the density of STS coverage along the Y chromosome, reducing the average spacing to 120 kb assuming a length of 30 Mb for the euchromatin. The average size of 61 YACs determined by pulsed-field gel electrophoresis analysis was at least 0.9 Mb. Minor differences noted between the ordering of STSs on this map compared with those previously reported may be attributed to inherent polymorphism between the Y chromosomes used to construct the YAC libraries.

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KW - autosome

KW - euchromatin

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KW - molecular cloning

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KW - sequence tagged site

KW - Y chromosome

KW - yeast artificial chromosome

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SP - 266

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JF - Genomics

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ER -

A set of ninety-seven overlapping yeast artificial chromosome clones spanning the human Y chromosome euchromatin

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Keywords

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