A selective medium for quantitative reisolation of Trichoderma harzianum from Agaricus bisporus compost

J Williams, J M Clarkson, P R Mills, R M Cooper

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We adapted a selective medium, previously developed for reisolation of Trichoderma spp. from soil, for quantitative determination of growth of T. harzianum from commercial Agaricus bisporus composts. This medium enables comparisons of aggressive (sensu inhibition of A. bisporus yield) with nonaggressive T. harzianum groups. The resulting medium contains the antimicrobials chloramphenicol, streptomycin, quintozene, and propamocarb and was highly selective, allowing the recovery of T. harzianum, as viable conidia and hyphal fragments, in compact colonies with the absence of visible microbial contaminants.
Original languageEnglish
Pages (from-to)4190-4191
Number of pages2
JournalApplied and Environmental Microbiology
Volume69
Issue number7
DOIs
Publication statusPublished - 2003

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Agaricus
Trichoderma
Agaricus bisporus
Trichoderma harzianum
selective media
compost
composts
Soil
Fungal Spores
pollutant
Streptomycin
Chloramphenicol
propamocarb
quintozene
soil
microbial contamination
chloramphenicol
streptomycin
conidia
Growth

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A selective medium for quantitative reisolation of Trichoderma harzianum from Agaricus bisporus compost. / Williams, J; Clarkson, J M; Mills, P R; Cooper, R M.

In: Applied and Environmental Microbiology, Vol. 69, No. 7, 2003, p. 4190-4191.

Research output: Contribution to journalArticle

Williams, J ; Clarkson, J M ; Mills, P R ; Cooper, R M. / A selective medium for quantitative reisolation of Trichoderma harzianum from Agaricus bisporus compost. In: Applied and Environmental Microbiology. 2003 ; Vol. 69, No. 7. pp. 4190-4191.
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