TY - JOUR
T1 - A selective medium for quantitative reisolation of Trichoderma harzianum from Agaricus bisporus compost
AU - Williams, J
AU - Clarkson, J M
AU - Mills, P R
AU - Cooper, R M
N1 - ID number: ISI:000184082100066
PY - 2003
Y1 - 2003
N2 - We adapted a selective medium, previously developed for reisolation of Trichoderma spp. from soil, for quantitative determination of growth of T. harzianum from commercial Agaricus bisporus composts. This medium enables comparisons of aggressive (sensu inhibition of A. bisporus yield) with nonaggressive T. harzianum groups. The resulting medium contains the antimicrobials chloramphenicol, streptomycin, quintozene, and propamocarb and was highly selective, allowing the recovery of T. harzianum, as viable conidia and hyphal fragments, in compact colonies with the absence of visible microbial contaminants.
AB - We adapted a selective medium, previously developed for reisolation of Trichoderma spp. from soil, for quantitative determination of growth of T. harzianum from commercial Agaricus bisporus composts. This medium enables comparisons of aggressive (sensu inhibition of A. bisporus yield) with nonaggressive T. harzianum groups. The resulting medium contains the antimicrobials chloramphenicol, streptomycin, quintozene, and propamocarb and was highly selective, allowing the recovery of T. harzianum, as viable conidia and hyphal fragments, in compact colonies with the absence of visible microbial contaminants.
UR - http://dx.doi.org/10.1128/AEM.69.7.4190-4191.2003
U2 - 10.1128/AEM.69.7.4190-4191.2003
DO - 10.1128/AEM.69.7.4190-4191.2003
M3 - Article
SN - 0099-2240
VL - 69
SP - 4190
EP - 4191
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
IS - 7
ER -