Abstract
Objectives: Production of channelled, porous polycaprolactone (PCL) sheets that can be rolled into an X cm long and Y cm diameter cylindrical scaffold. The work provides a proof of concept for the use of channelled scaffolds as both a means of media supply and the scaffold for myoblast expansion and differentiation.
Methods: Channelled PCL sheets were produced by employing sheet casting methods by phase inversion [4]. NaCl particles were used as a porogen and water as the non-solvent and to leach the porogen. C2C12s were cultured in media (DMEM + 10% FBS) on 2D flat sheets, and 3D rolled sheets with flow through the channels and recycling.
Results: The PCL sheets were porous and the channels were intact, as observed on SEM images. The PCL sheet demonstrated good mechanical properties such that they could be rolled without damage. Fibrin was used as a hydrogel to achieve high cell seeding within PCL porous sheets. Attachment and proliferation of C2C12s was tested in 2D before, and 3D after rolling. Proliferation of C2C12s on the scaffold inside a perfusion reactor was assessed using histological methods.
Conclusions: Porous channelled PCL sheets support C2C12 culture, and can be rolled without damage, and utilised with flow as a scaffold within a perfusion bioreactor.
References:
1) Post, M., et al., New Aspects of Meat Quality, 2017, Woodhead Publishing, 425-441.
2) Rnjak-Kovacin, J., Kaplan, D., et al., Adv Funct Mater, 2014, 24 (15), 2188-2196.
3) Pourchet, L., Bioprinting, 2019, 13, e00039.
4) Iwasaki, K., Circulation, 2008,118 (14 Suppl), 52-57.
Acknowledgements: University of Bath for all kind helps including financial support.
Methods: Channelled PCL sheets were produced by employing sheet casting methods by phase inversion [4]. NaCl particles were used as a porogen and water as the non-solvent and to leach the porogen. C2C12s were cultured in media (DMEM + 10% FBS) on 2D flat sheets, and 3D rolled sheets with flow through the channels and recycling.
Results: The PCL sheets were porous and the channels were intact, as observed on SEM images. The PCL sheet demonstrated good mechanical properties such that they could be rolled without damage. Fibrin was used as a hydrogel to achieve high cell seeding within PCL porous sheets. Attachment and proliferation of C2C12s was tested in 2D before, and 3D after rolling. Proliferation of C2C12s on the scaffold inside a perfusion reactor was assessed using histological methods.
Conclusions: Porous channelled PCL sheets support C2C12 culture, and can be rolled without damage, and utilised with flow as a scaffold within a perfusion bioreactor.
References:
1) Post, M., et al., New Aspects of Meat Quality, 2017, Woodhead Publishing, 425-441.
2) Rnjak-Kovacin, J., Kaplan, D., et al., Adv Funct Mater, 2014, 24 (15), 2188-2196.
3) Pourchet, L., Bioprinting, 2019, 13, e00039.
4) Iwasaki, K., Circulation, 2008,118 (14 Suppl), 52-57.
Acknowledgements: University of Bath for all kind helps including financial support.
| Original language | English |
|---|---|
| Title of host publication | 5th International Scientific Conference on Cultured Meat |
| Publication status | Published - 7 Oct 2019 |