A role for topoisomerase i in Fusarium graminearum and F. culmorum pathogenesis and sporulation

Thomas K. Baldwin, Martin Urban, Neil Brown, Kim E. Hammond-Kosack

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Fusarium graminearum and F. culmorum are the causal agents of Fusarium ear blight (FEB) in wheat. A forward genetics approach was taken to discover novel pathogenicity genes in the genome of F. graminearum. A library of transformants created by random plasmid insertional mutagenesis was screened on wheat ears for virulence defects. Plasmid rescue on one of the reduced-virulence mutants revealed a single-copy plasmid insertion in the gene coding for the DNA interacting enzyme, topoisomerase I. Targeted topoisomerase I gene-deletion mutants were created in strains of both F. graminearum and F. culmorum. The top1 mutants of both species exhibited greatly reduced virulence in wheat ear infection assays (GO:0009405 and GO:0044145). Detailed microscopy analyses revealed that top1 hyphal growth was restricted to palea tissue whereas host responses were discernable 1,000 μm further away in the rachis node. Asexual sporulation was reduced in the F. graminearum mutants and was absent from the F. culmorum mutants. The F. graminearum mutant did not develop sexual spores when subjected to an in vitro perithecia production assay. During in vitro growth, the top1 mutants of both species were still able to produce the trichothecene mycotoxin, deoxynivalenol.

Original languageEnglish
Pages (from-to)566-577
Number of pages12
JournalMolecular Plant-Microbe Interactions
Volume23
Issue number5
DOIs
Publication statusPublished - 1 May 2010

Fingerprint

Fusarium culmorum
Fusarium graminearum
Fusarium
sporulation
Virulence
pathogenesis
Triticum
Ear
mutants
Type I DNA Topoisomerase
Plasmids
Insertional Mutagenesis
DNA topoisomerase
plasmids
Trichothecenes
virulence
Mycotoxins
Gene Deletion
wheat
Growth

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Physiology

Cite this

A role for topoisomerase i in Fusarium graminearum and F. culmorum pathogenesis and sporulation. / Baldwin, Thomas K.; Urban, Martin; Brown, Neil; Hammond-Kosack, Kim E.

In: Molecular Plant-Microbe Interactions, Vol. 23, No. 5, 01.05.2010, p. 566-577.

Research output: Contribution to journalArticle

Baldwin, Thomas K. ; Urban, Martin ; Brown, Neil ; Hammond-Kosack, Kim E. / A role for topoisomerase i in Fusarium graminearum and F. culmorum pathogenesis and sporulation. In: Molecular Plant-Microbe Interactions. 2010 ; Vol. 23, No. 5. pp. 566-577.
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