A restricted role for sperm-borne microRNAs in mammalian fertilization

M Amanai, M Brahmajosyula, Anthony C F Perry

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Prototypical microRNAs (miRNAs) are 21 similar to 25-base-pair RNAs that regulate differentiation, carcinogenesis, and pluripotency by eliminating mRNAs or blocking their translation, in a process that is collectively termed RNA interference (RNAi). In zebrafish, RNAi mediated by miRNAs regulates early development, and in mice embryos that lack the miRNA precursor processor Dicer are nonviable. However, the roles of miRNAs in mammalian fertilization are unknown. In this report, we show using microarrays that miRNAs are present in mouse sperm structures that enter the oocyte at fertilization. The sperm contained a broad profile of miRNAs and a subset of potential mRNA targets, which were expressed in fertilizable metaphase 11 (mII) oocytes. Oocytes contained transcripts for the RNA-induced silencing complex (RISC) catalytic subunit, EIF2C3 (formerly AGO3). However, the levels of sperm-borne miRNA (measured by quantitative PCR) were low relative to those of unfertilized mII oocytes, and fertilization did not alter the mII oocyte miRNA repertoire that included the most abundant sperm-borne miRNAs. Coinjection of mII oocytes with sperm heads plus anti-miRNAs to suppress miRNA function did not perturb pronuclear activation or preimplantation development. In contrast, nuclear transfer by microinjection altered the miRNA profile of enucleated oocytes. These data suggest that sperm-borne prototypical miRNAs play a limited role, if any, in mammalian fertilization or early preimplantation development.
LanguageEnglish
Pages877-884
Number of pages8
JournalBiology of Reproduction
Volume75
Issue number6
DOIs
StatusPublished - 2006

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MicroRNAs
Fertilization
Spermatozoa
Oocytes
RNA Interference
RNA-Induced Silencing Complex
Sperm Head
Messenger RNA
Microinjections
Zebrafish
Metaphase
Base Pairing
Catalytic Domain
Carcinogenesis
Embryonic Structures
RNA

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A restricted role for sperm-borne microRNAs in mammalian fertilization. / Amanai, M; Brahmajosyula, M; Perry, Anthony C F.

In: Biology of Reproduction, Vol. 75, No. 6, 2006, p. 877-884.

Research output: Contribution to journalArticle

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