A program of successive gene expression in mammalian one-cell embryos during first cell cycle

Research output: Contribution to conferencePoster

Abstract

In mammal, by fertilization, sperm and oocyte unite to produce a totipotent one-cell embryo.  The gamete-derived genomes are known to be transcriptionally silent until the onset of embryonic genome activation (EGA).  EGA is critical for development and establishment of totipotency, yet its timing and profile remain elusive in both human and mouse.  We here employed high resolution single cell RNA sequence on precisely synchronized mouse embryos and human healthy binuclear (2PN) one-cell embryos. Results showed that programmed succession of gene expression during the first cell cycle (one- to two cell embryo development) and its upstream regulators.  Furthermore, together with live-imaging analyses and immunocytochemistry, multiple aspects of results indicated a unique, non-canonical, features of the first embryonic cell division.  
Original languageEnglish
Publication statusPublished - 9 May 2023
EventCell Cycle Conference - The Francis Crick Institute in London, London, UK United Kingdom
Duration: 9 May 2023 → …

Conference

ConferenceCell Cycle Conference
Country/TerritoryUK United Kingdom
CityLondon
Period9/05/23 → …

Keywords

  • Cell Division
  • embryonic genome activation
  • totipotency
  • human embryo
  • RNA sequence
  • Cell Cycle

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