Abstract

We report the development of a Lab-on-PCB DNA diagnostic platform, exploiting peptide nucleic acid (PNA) sequences as probes. The study demonstrates the optimization and characterization of two commercial PCB manufacturing gold electroplating processes for biosensing applications. Using an optimized ratio of PNA with a spacer molecule (MCH), the lowest limit of detection (LoD) to date for PCB-based DNA biosensors of 57 fM is reported. The study also showcases a fully integrated Lab-on-PCB microsystem designed for rapid detection, which employs PCB-integrated sample delivery, achieving DNA quantification in the 0.1–100 pM range for 5 μL samples analyzed within 5 min under continuous flow. The demonstrated biosensor proves the capability of PCB-based DNA biosensors for high sensitivity and paves the way for their integration in Lab-on-PCB DNA diagnostic microsystems.
Original languageEnglish
Pages (from-to)244-250
Number of pages7
JournalBiosensors and Bioelectronics
Volume123
Early online date6 Sep 2018
DOIs
Publication statusPublished - 1 Jan 2019

Fingerprint

Peptide Nucleic Acids
Polychlorinated Biphenyls
Polychlorinated biphenyls
DNA
Biosensing Techniques
Biosensors
Microsystems
Nucleic acid sequences
Electroplating
Gold
Limit of Detection
Molecules

Keywords

  • Biosensor
  • DNA
  • Impedimetric
  • Nucleic acid
  • PCB
  • PNA

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

A PNA-based Lab-on-PCB diagnostic platform for rapid and high sensitivity DNA quantification. / Jolly, Pawan; Rainbow, Joshua; Regoutz, Anna ; Estrela, Pedro; Moschou, Despina.

In: Biosensors and Bioelectronics, Vol. 123, 01.01.2019, p. 244-250.

Research output: Contribution to journalArticle

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