A PNA-based Lab-on-PCB diagnostic platform for rapid and high sensitivity DNA quantification

Pawan Jolly; Joshua Rainbow; Anna  Regoutz; Pedro Estrela; Despina Moschou

doi:10.1016/j.bios.2018.09.006

A PNA-based Lab-on-PCB diagnostic platform for rapid and high sensitivity DNA quantification

Pawan Jolly, Joshua Rainbow, Anna Regoutz, Pedro Estrela, Despina Moschou

Research output: Contribution to journal › Article › peer-review

54 Citations (SciVal)

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Abstract

We report the development of a Lab-on-PCB DNA diagnostic platform, exploiting peptide nucleic acid (PNA) sequences as probes. The study demonstrates the optimization and characterization of two commercial PCB manufacturing gold electroplating processes for biosensing applications. Using an optimized ratio of PNA with a spacer molecule (MCH), the lowest limit of detection (LoD) to date for PCB-based DNA biosensors of 57 fM is reported. The study also showcases a fully integrated Lab-on-PCB microsystem designed for rapid detection, which employs PCB-integrated sample delivery, achieving DNA quantification in the 0.1–100 pM range for 5 μL samples analyzed within 5 min under continuous flow. The demonstrated biosensor proves the capability of PCB-based DNA biosensors for high sensitivity and paves the way for their integration in Lab-on-PCB DNA diagnostic microsystems.

Original language	English
Pages (from-to)	244-250
Number of pages	7
Journal	Biosensors and Bioelectronics
Volume	123
Early online date	6 Sept 2018
DOIs	https://doi.org/10.1016/j.bios.2018.09.006
Publication status	Published - 1 Jan 2019

Keywords

Biosensor
DNA
Impedimetric
Nucleic acid
PCB
PNA

ASJC Scopus subject areas

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

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10.1016/j.bios.2018.09.006

Manuscript_final_revisedAccepted author manuscript, 763 KBLicence: CC BY-NC-ND

Cite this

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title = "A PNA-based Lab-on-PCB diagnostic platform for rapid and high sensitivity DNA quantification",

abstract = "We report the development of a Lab-on-PCB DNA diagnostic platform, exploiting peptide nucleic acid (PNA) sequences as probes. The study demonstrates the optimization and characterization of two commercial PCB manufacturing gold electroplating processes for biosensing applications. Using an optimized ratio of PNA with a spacer molecule (MCH), the lowest limit of detection (LoD) to date for PCB-based DNA biosensors of 57 fM is reported. The study also showcases a fully integrated Lab-on-PCB microsystem designed for rapid detection, which employs PCB-integrated sample delivery, achieving DNA quantification in the 0.1–100 pM range for 5 μL samples analyzed within 5 min under continuous flow. The demonstrated biosensor proves the capability of PCB-based DNA biosensors for high sensitivity and paves the way for their integration in Lab-on-PCB DNA diagnostic microsystems.",

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author = "Pawan Jolly and Joshua Rainbow and Anna Regoutz and Pedro Estrela and Despina Moschou",

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AU - Jolly, Pawan

AU - Rainbow, Joshua

AU - Regoutz, Anna

AU - Estrela, Pedro

AU - Moschou, Despina

PY - 2019/1/1

Y1 - 2019/1/1

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KW - Impedimetric

KW - Nucleic acid

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A PNA-based Lab-on-PCB diagnostic platform for rapid and high sensitivity DNA quantification

Abstract

Keywords

ASJC Scopus subject areas

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Pedro Estrela

Despina Moschou

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A PNA-based Lab-on-PCB diagnostic platform for rapid and high sensitivity DNA quantification

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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Fingerprint

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Pedro Estrela

Despina Moschou

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