A plant immune receptor detects pathogen effectors that target WRKY transcription factors

Panagiotis F Sarris, Zane Duxbury, Sung Un Huh, Yan Ma, Cécile Segonzac, Jan Sklenar, Paul Derbyshire, Volkan Cevik, Ghanasyam Rallapalli, Simon B Saucet, Lennart Wirthmueller, Frank L H Menke, Kee Hoon Sohn, Jonathan D G Jones

Research output: Contribution to journalArticle

163 Citations (Scopus)

Abstract

Defense against pathogens in multicellular eukaryotes depends on intracellular immune receptors, yet surveillance by these receptors is poorly understood. Several plant nucleotide-binding, leucine-rich repeat (NB-LRR) immune receptors carry fusions with other protein domains. The Arabidopsis RRS1-R NB-LRR protein carries a C-terminal WRKY DNA binding domain and forms a receptor complex with RPS4, another NB-LRR protein. This complex detects the bacterial effectors AvrRps4 or PopP2 and then activates defense. Both bacterial proteins interact with the RRS1 WRKY domain, and PopP2 acetylates lysines to block DNA binding. PopP2 and AvrRps4 interact with other WRKY domain-containing proteins, suggesting these effectors interfere with WRKY transcription factor-dependent defense, and RPS4/RRS1 has integrated a "decoy" domain that enables detection of effectors that target WRKY proteins. We propose that NB-LRR receptor pairs, one member of which carries an additional protein domain, enable perception of pathogen effectors whose function is to target that domain.

LanguageEnglish
Pages1089-1100
Number of pages12
JournalCell
Volume161
Issue number5
Early online date21 May 2015
DOIs
StatusE-pub ahead of print - 21 May 2015

Fingerprint

Pathogens
Transcription Factors
Nucleotides
Leucine
Proteins
Bacterial Proteins
DNA
Eukaryota
Arabidopsis
Lysine
Fusion reactions
Protein Domains
leucine-rich repeat proteins

Keywords

  • Amino Acid Sequence
  • Arabidopsis
  • Arabidopsis Proteins
  • Bacterial Proteins
  • Immunity, Innate
  • Molecular Sequence Data
  • Plant Proteins
  • Protein Structure, Tertiary
  • Pseudomonas fluorescens
  • Pseudomonas syringae
  • Tobacco
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

Sarris, P. F., Duxbury, Z., Huh, S. U., Ma, Y., Segonzac, C., Sklenar, J., ... Jones, J. D. G. (2015). A plant immune receptor detects pathogen effectors that target WRKY transcription factors. Cell, 161(5), 1089-1100. https://doi.org/10.1016/j.cell.2015.04.024

A plant immune receptor detects pathogen effectors that target WRKY transcription factors. / Sarris, Panagiotis F; Duxbury, Zane; Huh, Sung Un; Ma, Yan; Segonzac, Cécile; Sklenar, Jan; Derbyshire, Paul; Cevik, Volkan; Rallapalli, Ghanasyam; Saucet, Simon B; Wirthmueller, Lennart; Menke, Frank L H; Sohn, Kee Hoon; Jones, Jonathan D G.

In: Cell, Vol. 161, No. 5, 21.05.2015, p. 1089-1100.

Research output: Contribution to journalArticle

Sarris, PF, Duxbury, Z, Huh, SU, Ma, Y, Segonzac, C, Sklenar, J, Derbyshire, P, Cevik, V, Rallapalli, G, Saucet, SB, Wirthmueller, L, Menke, FLH, Sohn, KH & Jones, JDG 2015, 'A plant immune receptor detects pathogen effectors that target WRKY transcription factors', Cell, vol. 161, no. 5, pp. 1089-1100. https://doi.org/10.1016/j.cell.2015.04.024
Sarris PF, Duxbury Z, Huh SU, Ma Y, Segonzac C, Sklenar J et al. A plant immune receptor detects pathogen effectors that target WRKY transcription factors. Cell. 2015 May 21;161(5):1089-1100. https://doi.org/10.1016/j.cell.2015.04.024
Sarris, Panagiotis F ; Duxbury, Zane ; Huh, Sung Un ; Ma, Yan ; Segonzac, Cécile ; Sklenar, Jan ; Derbyshire, Paul ; Cevik, Volkan ; Rallapalli, Ghanasyam ; Saucet, Simon B ; Wirthmueller, Lennart ; Menke, Frank L H ; Sohn, Kee Hoon ; Jones, Jonathan D G. / A plant immune receptor detects pathogen effectors that target WRKY transcription factors. In: Cell. 2015 ; Vol. 161, No. 5. pp. 1089-1100.
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AU - Sklenar, Jan

AU - Derbyshire, Paul

AU - Cevik, Volkan

AU - Rallapalli, Ghanasyam

AU - Saucet, Simon B

AU - Wirthmueller, Lennart

AU - Menke, Frank L H

AU - Sohn, Kee Hoon

AU - Jones, Jonathan D G

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N2 - Defense against pathogens in multicellular eukaryotes depends on intracellular immune receptors, yet surveillance by these receptors is poorly understood. Several plant nucleotide-binding, leucine-rich repeat (NB-LRR) immune receptors carry fusions with other protein domains. The Arabidopsis RRS1-R NB-LRR protein carries a C-terminal WRKY DNA binding domain and forms a receptor complex with RPS4, another NB-LRR protein. This complex detects the bacterial effectors AvrRps4 or PopP2 and then activates defense. Both bacterial proteins interact with the RRS1 WRKY domain, and PopP2 acetylates lysines to block DNA binding. PopP2 and AvrRps4 interact with other WRKY domain-containing proteins, suggesting these effectors interfere with WRKY transcription factor-dependent defense, and RPS4/RRS1 has integrated a "decoy" domain that enables detection of effectors that target WRKY proteins. We propose that NB-LRR receptor pairs, one member of which carries an additional protein domain, enable perception of pathogen effectors whose function is to target that domain.

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