A Photodeactivatable Antagonist for Controlling CREB-Dependent Gene Expression

Takuma Imoto, Masafumi Minoshima, Tatsushi Yokoyama, Ben P. Emery, Steven D. Bull, Haruhiko Bito, Kazuya Kikuchi

Research output: Contribution to journalArticle

Abstract

A novel photodeactivation strategy for controlling gene expression has been developed based on light-induced activation of cAMP response element binding protein (CREB). Light-induced cleavage of the photoresponsive protecting group of an antagonist of CREB binding protein (CBP) results in photocleaved products with weak binding affinity for CBP. This photodissociation reaction enables protein-protein interactions between CBP and CREB that trigger the formation of a multiprotein transcription complex to turn gene expression "on". This enables irradiation of antagonist-treated HEK293T cells to be used to trigger temporal recovery of CREB-dependent transcriptional activity and endogenous gene expression under photolytic control.

Original languageEnglish
JournalACS Central Science
DOIs
Publication statusE-pub ahead of print - 31 Aug 2020

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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