A Photodeactivatable Antagonist for Controlling CREB-Dependent Gene Expression

Takuma Imoto; Masafumi Minoshima; Tatsushi Yokoyama; Ben P. Emery; Steven D. Bull; Haruhiko Bito; Kazuya Kikuchi

doi:10.1021/acscentsci.0c00736

A Photodeactivatable Antagonist for Controlling CREB-Dependent Gene Expression

Takuma Imoto, Masafumi Minoshima, Tatsushi Yokoyama, Ben P. Emery, Steven D. Bull, Haruhiko Bito, Kazuya Kikuchi

Research output: Contribution to journal › Article

Abstract

A novel photodeactivation strategy for controlling gene expression has been developed based on light-induced activation of cAMP response element binding protein (CREB). Light-induced cleavage of the photoresponsive protecting group of an antagonist of CREB binding protein (CBP) results in photocleaved products with weak binding affinity for CBP. This photodissociation reaction enables protein-protein interactions between CBP and CREB that trigger the formation of a multiprotein transcription complex to turn gene expression "on". This enables irradiation of antagonist-treated HEK293T cells to be used to trigger temporal recovery of CREB-dependent transcriptional activity and endogenous gene expression under photolytic control.

Original language	English
Journal	ACS Central Science
DOIs	https://doi.org/10.1021/acscentsci.0c00736
Publication status	E-pub ahead of print - 31 Aug 2020

ASJC Scopus subject areas

Chemistry(all)
Chemical Engineering(all)

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10.1021/acscentsci.0c00736

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abstract = "A novel photodeactivation strategy for controlling gene expression has been developed based on light-induced activation of cAMP response element binding protein (CREB). Light-induced cleavage of the photoresponsive protecting group of an antagonist of CREB binding protein (CBP) results in photocleaved products with weak binding affinity for CBP. This photodissociation reaction enables protein-protein interactions between CBP and CREB that trigger the formation of a multiprotein transcription complex to turn gene expression {"}on{"}. This enables irradiation of antagonist-treated HEK293T cells to be used to trigger temporal recovery of CREB-dependent transcriptional activity and endogenous gene expression under photolytic control.",

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language = "English",

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AU - Yokoyama, Tatsushi

AU - Emery, Ben P.

AU - Bull, Steven D.

AU - Bito, Haruhiko

AU - Kikuchi, Kazuya

PY - 2020/8/31

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AB - A novel photodeactivation strategy for controlling gene expression has been developed based on light-induced activation of cAMP response element binding protein (CREB). Light-induced cleavage of the photoresponsive protecting group of an antagonist of CREB binding protein (CBP) results in photocleaved products with weak binding affinity for CBP. This photodissociation reaction enables protein-protein interactions between CBP and CREB that trigger the formation of a multiprotein transcription complex to turn gene expression "on". This enables irradiation of antagonist-treated HEK293T cells to be used to trigger temporal recovery of CREB-dependent transcriptional activity and endogenous gene expression under photolytic control.

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