A passive micromixer for enzymatic digestion of DNA

V. E. Papadopoulos, I. N. Kefala, G. Kaprou, G. Kokkoris, D. Moschou, G. Papadakis, E. Gizeli, A. Tserepi

Research output: Contribution to journalArticlepeer-review

43 Citations (SciVal)


A passive micromixer with zigzag geometry is demonstrated in this work to perform simultaneously mixing of a restriction enzyme with DNA and digestion of DNA. The total length required for complete mixing is estimated by simulation. The micromixer is fabricated from an imide-based photoimageable dry-film using flexible printed circuit technology that allows easy integration of the microdevice in more complex Lab-on-a-Chip (LoC) platforms. When heated at 37 °C, the micromixer achieves both complete mixing of the reagents and DNA digestion with restriction enzymes within 2.5 min, i.e., a time comparable to the incubation step needed in conventional digestion systems. Thus, it renders further incubation unnecessary and is proved a valuable component of LoC systems for diagnostic purposes.

Original languageEnglish
Pages (from-to)42-46
Number of pages5
JournalMicroelectronic Engineering
Publication statusPublished - 25 Jul 2014


  • DNA digestion
  • Flexible printed circuit
  • Lab on a Chip (LoC)
  • Microfluidics
  • Passive micromixer

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Electrical and Electronic Engineering


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