Abstract
A passive micromixer with zigzag geometry is demonstrated in this work to perform simultaneously mixing of a restriction enzyme with DNA and digestion of DNA. The total length required for complete mixing is estimated by simulation. The micromixer is fabricated from an imide-based photoimageable dry-film using flexible printed circuit technology that allows easy integration of the microdevice in more complex Lab-on-a-Chip (LoC) platforms. When heated at 37 °C, the micromixer achieves both complete mixing of the reagents and DNA digestion with restriction enzymes within 2.5 min, i.e., a time comparable to the incubation step needed in conventional digestion systems. Thus, it renders further incubation unnecessary and is proved a valuable component of LoC systems for diagnostic purposes.
Original language | English |
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Pages (from-to) | 42-46 |
Number of pages | 5 |
Journal | Microelectronic Engineering |
Volume | 124 |
DOIs | |
Publication status | Published - 25 Jul 2014 |
Keywords
- DNA digestion
- Flexible printed circuit
- Lab on a Chip (LoC)
- Microfluidics
- Passive micromixer
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Condensed Matter Physics
- Surfaces, Coatings and Films
- Electrical and Electronic Engineering