Projects per year
Abstract
A new label-free electrochemical DNA (E-DNA) biosensor using a custom synthesized ferrocenyl (Fc) double-stranded DNA intercalator as a redox marker is presented. Single-stranded DNA (ssDNA) was co-immobilized on gold electrodes with 6-mecarpto-hexanol to control the surface density of the ssDNA probe, and hybridized with complementary DNA. The binding of the Fc intercalator to dsDNA was measured by differential pulse voltammetry. This new biosensor was optimized to allow the detection of single base pair mismatched sequences, able to detect as low as 10 pM target ssDNA with a dynamic range from 10 pM to 100 nM. DNA extracted from wastewater was analyzed by quantitative polymerase chain reaction targeting human-specific mitochondrial DNA (mtDNA). The aim of this approach is to enable the analysis of population biomarkers in wastewater for the evaluation of public health using wastewater-based epidemiology (WBE). The E-DNA biosensor was employed to detect human-specific mtDNA from wastewater before and after PCR amplification. The results demonstrate the feasibility of detecting human DNA biomarkers in wastewater using the developed biosensor, which may allow the further development of DNA population biomarkers for public health using WBE.
| Original language | English |
|---|---|
| Pages (from-to) | 5609-5617 |
| Number of pages | 9 |
| Journal | Environmental Science & Technology |
| Volume | 49 |
| Issue number | 9 |
| Early online date | 23 Apr 2015 |
| DOIs | |
| Publication status | Published - 5 May 2015 |
Access to Document
- manuscript_R.PDF
This document is the Accepted Manuscript version of a Published Work that appeared in final form in Environmental Science & Technology, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/doi/10.1021/acs.est.5b00637.
Fingerprint
Dive into the research topics of 'A novel DNA biosensor using a ferrocenyl intercalator applied to the potential detection of human population biomarkers in wastewater'. Together they form a unique fingerprint.Projects
- 2 Finished
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Marie Curie ITN - SEWPROF A New Paradigm in Drug Use and Human Health Risk Assessment: Sewage Profiling at the Community Level management
Kasprzyk-Hordern, B. (PI), Estrela, P. (CoI) & Frost, C. (CoI)
1/10/12 → 30/09/16
Project: EU Commission
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Cancer Diagnosis: Parallel Sensing of Prostate Cancer Biomarkers: MARIE CURIE - PROSENSE Training budget
Estrela, P. (PI), Eggleston, I. (CoI), Frost, C. (CoI), Lloyd, M. (CoI), Pascu, S. (CoI) & Tyrrell, R. (CoI)
1/10/12 → 30/09/16
Project: EU Commission
Profiles
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Pedro Estrela
- Department of Electronic & Electrical Engineering - Professor
- Centre for Bioengineering & Biomedical Technologies (CBio) - Centre Director
- Centre for Therapeutic Innovation
- Centre for Sustainable Chemical Technologies (CSCT)
- Water Innovation and Research Centre (WIRC)
- Centre for Nanoscience and Nanotechnology
- Institute of Sustainability and Climate Change
- Centre for Integrated Materials, Processes & Structures (IMPS)
- Centre for Regenerative Design & Engineering for a Net Positive World (RENEW)
- Bath Institute for the Augmented Human
- Centre of Excellence in Water-Based Early-Warning Systems for Health Protection (CWBE)
Person: Research & Teaching, Core staff, Affiliate staff
Equipment
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MC2- Nuclear Magnetic Resonance (NMR)
Material and Chemical Characterisation (MC2)Facility/equipment: Technology type