A novel colorimetric assay for α-methylacyl-CoA racemase 1A (AMACR; P504S) utilizing the elimination of 2,4-dinitrophenolate

Maksims Jevglevskis, Guat Ling Lee, Amit Nathubhai, Yoana Petrova, Tony James, Michael Threadgill, Timothy Woodman, Matthew Lloyd

Research output: Contribution to journalArticle

6 Citations (Scopus)


α-Methylacyl-CoA racemase (AMACR; P504S) regulates branched-chain fatty acid degradation, activates Ibuprofen and is a recognised cancer drug target. A novel, facile colorimetric assay was developed based on elimination of 2,4-dinitrophenolate. The assay was used to test 5 known inhibitors, determining IC50 and Ki values, reversibility and characterize irreversible inhibition.
Original languageEnglish
Pages (from-to)5087-5090
Number of pages4
JournalChemical Communications
Issue number37
Early online date17 Mar 2017
Publication statusPublished - 7 May 2017


Cite this