A novel colorimetric assay for α-methylacyl-CoA racemase 1A (AMACR; P504S) utilizing the elimination of 2,4-dinitrophenolate

Maksims Jevglevskis, Guat Ling Lee, Amit Nathubhai, Yoana Petrova, Tony James, Michael Threadgill, Timothy Woodman, Matthew Lloyd

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

α-Methylacyl-CoA racemase (AMACR; P504S) regulates branched-chain fatty acid degradation, activates Ibuprofen and is a recognised cancer drug target. A novel, facile colorimetric assay was developed based on elimination of 2,4-dinitrophenolate. The assay was used to test 5 known inhibitors, determining IC50 and Ki values, reversibility and characterize irreversible inhibition.
Original languageEnglish
Pages (from-to)5087-5090
Number of pages4
JournalChemical Communications
Volume53
Issue number37
Early online date17 Mar 2017
DOIs
Publication statusPublished - 7 May 2017

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Assays
Ibuprofen
Fatty acids
Fatty Acids
Degradation
Pharmaceutical Preparations
alpha-methylacyl-CoA racemase

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A novel colorimetric assay for α-methylacyl-CoA racemase 1A (AMACR; P504S) utilizing the elimination of 2,4-dinitrophenolate. / Jevglevskis, Maksims; Lee, Guat Ling; Nathubhai, Amit; Petrova, Yoana; James, Tony; Threadgill, Michael; Woodman, Timothy; Lloyd, Matthew.

In: Chemical Communications, Vol. 53, No. 37, 07.05.2017, p. 5087-5090.

Research output: Contribution to journalArticle

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