α-Methylacyl-CoA racemase (AMACR; P504S) regulates branched-chain fatty acid degradation, activates Ibuprofen and is a recognised cancer drug target. A novel, facile colorimetric assay was developed based on elimination of 2,4-dinitrophenolate. The assay was used to test 5 known inhibitors, determining IC50 and Ki values, reversibility and characterize irreversible inhibition.
Jevglevskis, M., Lee, G. L., Nathubhai, A., Petrova, Y., James, T., Threadgill, M., ... Lloyd, M. (2017). A novel colorimetric assay for α-methylacyl-CoA racemase 1A (AMACR; P504S) utilizing the elimination of 2,4-dinitrophenolate. Chemical Communications, 53(37), 5087-5090. https://doi.org/10.1039/C7CC00476A