A novel colorimetric assay for α-methylacyl-CoA racemase 1A (AMACR; P504S) utilizing the elimination of 2,4-dinitrophenolate

Maksims Jevglevskis; Guat Ling Lee; Amit Nathubhai; Yoana Petrova; Tony James; Michael Threadgill; Timothy Woodman; Matthew Lloyd

doi:10.1039/C7CC00476A

A novel colorimetric assay for α-methylacyl-CoA racemase 1A (AMACR; P504S) utilizing the elimination of 2,4-dinitrophenolate

Maksims Jevglevskis, Guat Ling Lee, Amit Nathubhai, Yoana Petrova, Tony James, Michael Threadgill, Timothy Woodman, Matthew Lloyd

Research output: Contribution to journal › Article › peer-review

18 Citations (SciVal)

Abstract

α-Methylacyl-CoA racemase (AMACR; P504S) regulates branched-chain fatty acid degradation, activates Ibuprofen and is a recognised cancer drug target. A novel, facile colorimetric assay was developed based on elimination of 2,4-dinitrophenolate. The assay was used to test 5 known inhibitors, determining IC₅₀ and K_i values, reversibility and characterize irreversible inhibition.

Original language	English
Pages (from-to)	5087-5090
Number of pages	4
Journal	Chemical Communications
Volume	53
Issue number	37
Early online date	17 Mar 2017
DOIs	https://doi.org/10.1039/C7CC00476A
Publication status	Published - 7 May 2017

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1039/C7CC00476ALicence: CC BY

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abstract = "α-Methylacyl-CoA racemase (AMACR; P504S) regulates branched-chain fatty acid degradation, activates Ibuprofen and is a recognised cancer drug target. A novel, facile colorimetric assay was developed based on elimination of 2,4-dinitrophenolate. The assay was used to test 5 known inhibitors, determining IC50 and Ki values, reversibility and characterize irreversible inhibition. ",

author = "Maksims Jevglevskis and Lee, {Guat Ling} and Amit Nathubhai and Yoana Petrova and Tony James and Michael Threadgill and Timothy Woodman and Matthew Lloyd",

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AU - Jevglevskis, Maksims

AU - Lee, Guat Ling

AU - Nathubhai, Amit

AU - Petrova, Yoana

AU - James, Tony

AU - Threadgill, Michael

AU - Woodman, Timothy

AU - Lloyd, Matthew

PY - 2017/5/7

Y1 - 2017/5/7

N2 - α-Methylacyl-CoA racemase (AMACR; P504S) regulates branched-chain fatty acid degradation, activates Ibuprofen and is a recognised cancer drug target. A novel, facile colorimetric assay was developed based on elimination of 2,4-dinitrophenolate. The assay was used to test 5 known inhibitors, determining IC50 and Ki values, reversibility and characterize irreversible inhibition.

AB - α-Methylacyl-CoA racemase (AMACR; P504S) regulates branched-chain fatty acid degradation, activates Ibuprofen and is a recognised cancer drug target. A novel, facile colorimetric assay was developed based on elimination of 2,4-dinitrophenolate. The assay was used to test 5 known inhibitors, determining IC50 and Ki values, reversibility and characterize irreversible inhibition.

UR - https://doi.org/10.1039/C7CC00476A

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DO - 10.1039/C7CC00476A

M3 - Article

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JO - Chemical Communications

JF - Chemical Communications

IS - 37

ER -

A novel colorimetric assay for α-methylacyl-CoA racemase 1A (AMACR; P504S) utilizing the elimination of 2,4-dinitrophenolate

Abstract

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AMACR Targeted Drugs for Treating Advance Prostate Cancer

AMACR Targeted Drugs for Treating Advance Prostate Cancer

Matthew Lloyd

Avance III 500 MHz Nuclear Magnetic Resonance (NMR) Spectrometer (9West)

MC2- Nuclear Magnetic Resonance (NMR)

Cite this

A novel colorimetric assay for α-methylacyl-CoA racemase 1A (AMACR; P504S) utilizing the elimination of 2,4-dinitrophenolate

Abstract

UN SDGs

Access to Document

Other files and links

Fingerprint

Projects

AMACR Targeted Drugs for Treating Advance Prostate Cancer

AMACR Targeted Drugs for Treating Advance Prostate Cancer

Profiles

Matthew Lloyd

Equipment

Avance III 500 MHz Nuclear Magnetic Resonance (NMR) Spectrometer (9West)

MC2- Nuclear Magnetic Resonance (NMR)

Cite this