A novel cobalt complex for enhancing amperometric and impedimetric DNA detection

Edward M. Regan; Andrew J. Hallett; L. C. Caleb Wong; Ibrahim Q. Saeed; Emily E. Langdon-Jones; Niklaas J. Buurma; Simon J. A. Pope; Pedro Estrela

doi:10.1016/j.electacta.2013.10.028

A novel cobalt complex for enhancing amperometric and impedimetric DNA detection

Edward M. Regan, Andrew J. Hallett, L. C. Caleb Wong, Ibrahim Q. Saeed, Emily E. Langdon-Jones, Niklaas J. Buurma, Simon J. A. Pope, Pedro Estrela

Department of Electronic & Electrical Engineering

Research output: Contribution to journal › Article › peer-review

7 Citations (SciVal)

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Abstract

In this work we present a novel cobalt complex, [Co(GA)2(aqphen)]Cl that is water-soluble, redox-active and binds to dsDNA. We report that this complex can be used as a signal enhancer when detecting DNA hybridisation using electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). The compound mediates its EIS signal enhancement by causing an increase in charge transfer resistance (Rct) when bound to dsDNA. Increased peak currents are also observed with DPV when the compound is incubated with dsDNA as compared with ssDNA. We believe that this compound intercalates specifically with dsDNA and alters the DNA structure to affect the electrostatic barrier to charged redox markers in solution. To our knowledge this is the first example of a single compound that can enhance both amperometric and impedimetric signals for DNA detection. Our findings enable the development of a label-free and multi-modal approach to improve the sensitivity, accuracy and speed of electrochemical DNA detection.

Original language	English
Pages (from-to)	10-15
Number of pages	6
Journal	Electrochimica Acta
Volume	128
Early online date	18 Oct 2013
DOIs	https://doi.org/10.1016/j.electacta.2013.10.028
Publication status	Published - 10 May 2014

Keywords

DNA sensors
intercalators
electrochemical impedance spectroscopy
differential pulse voltammetry

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10.1016/j.electacta.2013.10.028

author's accepted version
NOTICE: this is the author’s version of a work that was accepted for publication in Electrochimica Acta. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Electrochimica Acta, V.128. May 2014. DOI: 10.1016/j.electacta.2013.10.028
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Cite this

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title = "A novel cobalt complex for enhancing amperometric and impedimetric DNA detection",

abstract = "In this work we present a novel cobalt complex, [Co(GA)2(aqphen)]Cl that is water-soluble, redox-active and binds to dsDNA. We report that this complex can be used as a signal enhancer when detecting DNA hybridisation using electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). The compound mediates its EIS signal enhancement by causing an increase in charge transfer resistance (Rct) when bound to dsDNA. Increased peak currents are also observed with DPV when the compound is incubated with dsDNA as compared with ssDNA. We believe that this compound intercalates specifically with dsDNA and alters the DNA structure to affect the electrostatic barrier to charged redox markers in solution. To our knowledge this is the first example of a single compound that can enhance both amperometric and impedimetric signals for DNA detection. Our findings enable the development of a label-free and multi-modal approach to improve the sensitivity, accuracy and speed of electrochemical DNA detection.",

keywords = "DNA sensors, intercalators, electrochemical impedance spectroscopy, differential pulse voltammetry",

author = "Regan, {Edward M.} and Hallett, {Andrew J.} and Wong, {L. C. Caleb} and Saeed, {Ibrahim Q.} and Langdon-Jones, {Emily E.} and Buurma, {Niklaas J.} and Pope, {Simon J. A.} and Pedro Estrela",

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AU - Regan, Edward M.

AU - Hallett, Andrew J.

AU - Wong, L. C. Caleb

AU - Saeed, Ibrahim Q.

AU - Langdon-Jones, Emily E.

AU - Buurma, Niklaas J.

AU - Pope, Simon J. A.

AU - Estrela, Pedro

PY - 2014/5/10

Y1 - 2014/5/10

N2 - In this work we present a novel cobalt complex, [Co(GA)2(aqphen)]Cl that is water-soluble, redox-active and binds to dsDNA. We report that this complex can be used as a signal enhancer when detecting DNA hybridisation using electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). The compound mediates its EIS signal enhancement by causing an increase in charge transfer resistance (Rct) when bound to dsDNA. Increased peak currents are also observed with DPV when the compound is incubated with dsDNA as compared with ssDNA. We believe that this compound intercalates specifically with dsDNA and alters the DNA structure to affect the electrostatic barrier to charged redox markers in solution. To our knowledge this is the first example of a single compound that can enhance both amperometric and impedimetric signals for DNA detection. Our findings enable the development of a label-free and multi-modal approach to improve the sensitivity, accuracy and speed of electrochemical DNA detection.

AB - In this work we present a novel cobalt complex, [Co(GA)2(aqphen)]Cl that is water-soluble, redox-active and binds to dsDNA. We report that this complex can be used as a signal enhancer when detecting DNA hybridisation using electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). The compound mediates its EIS signal enhancement by causing an increase in charge transfer resistance (Rct) when bound to dsDNA. Increased peak currents are also observed with DPV when the compound is incubated with dsDNA as compared with ssDNA. We believe that this compound intercalates specifically with dsDNA and alters the DNA structure to affect the electrostatic barrier to charged redox markers in solution. To our knowledge this is the first example of a single compound that can enhance both amperometric and impedimetric signals for DNA detection. Our findings enable the development of a label-free and multi-modal approach to improve the sensitivity, accuracy and speed of electrochemical DNA detection.

KW - DNA sensors

KW - intercalators

KW - electrochemical impedance spectroscopy

KW - differential pulse voltammetry

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JO - Electrochimica Acta

JF - Electrochimica Acta

ER -

A novel cobalt complex for enhancing amperometric and impedimetric DNA detection

Abstract

Keywords

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Enhancing Electrochemical DNA Biosensing with Active Sensitisers

Additional Sponsorship 20/11/12 in Support of EPSRC Delivery Plan

Pedro Estrela

Cite this

A novel cobalt complex for enhancing amperometric and impedimetric DNA detection

Abstract

Keywords

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Enhancing Electrochemical DNA Biosensing with Active Sensitisers

Additional Sponsorship 20/11/12 in Support of EPSRC Delivery Plan

Profiles

Pedro Estrela

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