Projects per year
Abstract
In this work we present a novel cobalt complex, [Co(GA)2(aqphen)]Cl that is water-soluble, redox-active and binds to dsDNA. We report that this complex can be used as a signal enhancer when detecting DNA hybridisation using electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). The compound mediates its EIS signal enhancement by causing an increase in charge transfer resistance (Rct) when bound to dsDNA. Increased peak currents are also observed with DPV when the compound is incubated with dsDNA as compared with ssDNA. We believe that this compound intercalates specifically with dsDNA and alters the DNA structure to affect the electrostatic barrier to charged redox markers in solution. To our knowledge this is the first example of a single compound that can enhance both amperometric and impedimetric signals for DNA detection. Our findings enable the development of a label-free and multi-modal approach to improve the sensitivity, accuracy and speed of electrochemical DNA detection.
Original language | English |
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Pages (from-to) | 10-15 |
Number of pages | 6 |
Journal | Electrochimica Acta |
Volume | 128 |
Early online date | 18 Oct 2013 |
DOIs | |
Publication status | Published - 10 May 2014 |
Keywords
- DNA sensors
- intercalators
- electrochemical impedance spectroscopy
- differential pulse voltammetry
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Dive into the research topics of 'A novel cobalt complex for enhancing amperometric and impedimetric DNA detection'. Together they form a unique fingerprint.Projects
- 2 Finished
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Enhancing Electrochemical DNA Biosensing with Active Sensitisers
Estrela, P. (PI) & Frost, C. (CoI)
Engineering and Physical Sciences Research Council
1/01/12 → 30/06/12
Project: Research council
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Additional Sponsorship 20/11/12 in Support of EPSRC Delivery Plan
Millar, J. (PI)
Engineering and Physical Sciences Research Council
1/07/11 → 30/06/12
Project: Research council
Profiles
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Pedro Estrela
- Department of Electronic & Electrical Engineering - Professor
- Centre for Bioengineering & Biomedical Technologies (CBio) - Centre Director
- Centre for Therapeutic Innovation
- Centre for Sustainable Chemical Technologies (CSCT)
- Water Innovation and Research Centre (WIRC)
- Centre for Nanoscience and Nanotechnology
- Institute of Sustainability and Climate Change
- Centre for Integrated Materials, Processes & Structures (IMPS)
- Centre for Regenerative Design & Engineering for a Net Positive World (RENEW)
- Bath Institute for the Augmented Human
- Centre of Excellence in Water-Based Early-Warning Systems for Health Protection (CWBE)
Person: Research & Teaching, Core staff, Affiliate staff