A novel cobalt complex for enhancing amperometric and impedimetric DNA detection

Edward M. Regan, Andrew J. Hallett, L. C. Caleb Wong, Ibrahim Q. Saeed, Emily E. Langdon-Jones, Niklaas J. Buurma, Simon J. A. Pope, Pedro Estrela

Research output: Contribution to journalArticle

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Abstract

In this work we present a novel cobalt complex, [Co(GA)2(aqphen)]Cl that is water-soluble, redox-active and binds to dsDNA. We report that this complex can be used as a signal enhancer when detecting DNA hybridisation using electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). The compound mediates its EIS signal enhancement by causing an increase in charge transfer resistance (Rct) when bound to dsDNA. Increased peak currents are also observed with DPV when the compound is incubated with dsDNA as compared with ssDNA. We believe that this compound intercalates specifically with dsDNA and alters the DNA structure to affect the electrostatic barrier to charged redox markers in solution. To our knowledge this is the first example of a single compound that can enhance both amperometric and impedimetric signals for DNA detection. Our findings enable the development of a label-free and multi-modal approach to improve the sensitivity, accuracy and speed of electrochemical DNA detection.
Original languageEnglish
Pages (from-to)10-15
Number of pages6
JournalElectrochimica Acta
Volume128
Early online date18 Oct 2013
DOIs
Publication statusPublished - 10 May 2014

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Cobalt
DNA
Voltammetry
Electrochemical impedance spectroscopy
Charge transfer
Labels
Electrostatics
Water
Oxidation-Reduction

Keywords

  • DNA sensors
  • intercalators
  • electrochemical impedance spectroscopy
  • differential pulse voltammetry

Cite this

Regan, E. M., Hallett, A. J., Wong, L. C. C., Saeed, I. Q., Langdon-Jones, E. E., Buurma, N. J., ... Estrela, P. (2014). A novel cobalt complex for enhancing amperometric and impedimetric DNA detection. Electrochimica Acta, 128, 10-15. https://doi.org/10.1016/j.electacta.2013.10.028

A novel cobalt complex for enhancing amperometric and impedimetric DNA detection. / Regan, Edward M.; Hallett, Andrew J.; Wong, L. C. Caleb; Saeed, Ibrahim Q.; Langdon-Jones, Emily E.; Buurma, Niklaas J.; Pope, Simon J. A.; Estrela, Pedro.

In: Electrochimica Acta, Vol. 128, 10.05.2014, p. 10-15.

Research output: Contribution to journalArticle

Regan, EM, Hallett, AJ, Wong, LCC, Saeed, IQ, Langdon-Jones, EE, Buurma, NJ, Pope, SJA & Estrela, P 2014, 'A novel cobalt complex for enhancing amperometric and impedimetric DNA detection', Electrochimica Acta, vol. 128, pp. 10-15. https://doi.org/10.1016/j.electacta.2013.10.028
Regan EM, Hallett AJ, Wong LCC, Saeed IQ, Langdon-Jones EE, Buurma NJ et al. A novel cobalt complex for enhancing amperometric and impedimetric DNA detection. Electrochimica Acta. 2014 May 10;128:10-15. https://doi.org/10.1016/j.electacta.2013.10.028
Regan, Edward M. ; Hallett, Andrew J. ; Wong, L. C. Caleb ; Saeed, Ibrahim Q. ; Langdon-Jones, Emily E. ; Buurma, Niklaas J. ; Pope, Simon J. A. ; Estrela, Pedro. / A novel cobalt complex for enhancing amperometric and impedimetric DNA detection. In: Electrochimica Acta. 2014 ; Vol. 128. pp. 10-15.
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