TY - JOUR
T1 - A new understanding of how temperature affects the catalytic activity of enzymes
AU - Daniel, R M
AU - Danson, Michael J
PY - 2010/10
Y1 - 2010/10
N2 - The two established thermal properties of enzymes are their activation energy and their thermal stability, but experimental data do not match the expectations of these two properties. The recently proposed Equilibrium Model (EM) provides a quantitative explanation of enzyme thermal behaviour under reaction conditions by introducing an inactive (but not denatured) intermediate in rapid equilibrium with the active form. It was formulated as a mathematical model, and fits the known experimental data. Importantly, the EM gives rise to a number of new insights into the molecular basis of the temperature control of enzymes and their environmental adaptation and evolution, it is consistent with active site properties, and it has fundamental implications for enzyme engineering and other areas of biotechnology.
AB - The two established thermal properties of enzymes are their activation energy and their thermal stability, but experimental data do not match the expectations of these two properties. The recently proposed Equilibrium Model (EM) provides a quantitative explanation of enzyme thermal behaviour under reaction conditions by introducing an inactive (but not denatured) intermediate in rapid equilibrium with the active form. It was formulated as a mathematical model, and fits the known experimental data. Importantly, the EM gives rise to a number of new insights into the molecular basis of the temperature control of enzymes and their environmental adaptation and evolution, it is consistent with active site properties, and it has fundamental implications for enzyme engineering and other areas of biotechnology.
UR - http://www.scopus.com/inward/record.url?scp=77957232344&partnerID=8YFLogxK
UR - http://dx.doi.org/10.1016/j.tibs.2010.05.001
U2 - 10.1016/j.tibs.2010.05.001
DO - 10.1016/j.tibs.2010.05.001
M3 - Article
VL - 35
SP - 584
EP - 591
JO - Trends in Biochemical Sciences
JF - Trends in Biochemical Sciences
SN - 0968-0004
IS - 10
ER -